IP Library › Granted Patent US 8,552,147
Granted Patent B2
US 8,552,147 · App. 13/142,358 · Granted Oct 8, 2013

Peptides and compositions for prevention of cell adhesion and methods of using same

Inventor: Amir Zlotkin (Tel Hashomer, IL)
Assignee: Tel Hashomer Medical Research, Infrastructure and Services Ltd.
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Quick Facts
Patent No.
US 8,552,147
App. No.
13/142,358
Granted
Oct 8, 2013
Kind
B2
Abstract

Compositions comprising an isolated peptide, which may for example optionally comprise a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3) or CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1), or any cyclized version thereof, and methods of using same, including for treatment of or prevention of formation of microbial biofilms and against adhesion of a cell to a surface.

Claims (33)

1. A cyclized peptide consisting of a length of up to 35 amino acids and comprising a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFDYDWY (SEQ ID NO: 7), SFDYDWYN (SEQ ID NO: 8), HSFDYDWYN (SEQ ID NO: 9), HSFDYDWYNV (SEQ ID NO: 10), VHSFDYDWYNV (SEQ ID NO: 11), VHSFDYDWYNVS (SEQ ID NO: 12), SVHSFDYDWYNVS (SEQ ID NO: 13), SVHSFDYDWYNVSD (SEQ ID NO: 14), KSVHSFDYDWYNVSD (SEQ ID NO: 15), KSVHSFDYDWYNVSDQ (SEQ ID NO: 16), NKSVHSFDYDWYNVSDQ (SEQ ID NO: 17), NKSVHSFDYDWYNVSDQA (SEQ ID NO: 18), QNKSVHSFDYDWYNVSDQA (SEQ ID NO: 19), QNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 20), SQNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 21), SQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 22), FSQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 23), FSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 24), SFSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 25), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3), CSFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 26), and CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1).

2. A composition comprising a cyclized peptide consisting of a length of up to 35 amino acids and comprising a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFDYDWY (SEQ ID NO: 7), SFDYDWYN (SEQ ID NO: 8), HSFDYDWYN (SEQ ID NO: 9), HSFDYDWYNV (SEQ ID NO: 10), VHSFDYDWYNV (SEQ ID NO: 11), VHSFDYDWYNVS (SEQ ID NO: 12), SVHSFDYDWYNVS (SEQ ID NO: 13), SVHSFDYDWYNVSD (SEQ ID NO: 14), KSVHSFDYDWYNVSD (SEQ ID NO: 15), KSVHSFDYDWYNVSDQ (SEQ ID NO: 16), NKSVHSFDYDWYNVSDQ (SEQ ID NO: 17), NKSVHSFDYDWYNVSDQA (SEQ ID NO: 18), QNKSVHSFDYDWYNVSDQA (SEQ ID NO: 19), QNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 20), SQNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 21), SQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 22), FSQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 23), FSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 24), SFSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 25), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3), CSFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 26) and CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1).

3. A method of inhibiting adhesion of a single cell organism to a surface, the method comprising contacting the cell with a composition comprising a cyclized peptide consisting of a length of up to 35 amino acids and comprising a sequence selected from the group consisting of FDYDWY (SEQ ID NO: 2), SFDYDWY (SEQ ID NO: 7), SFDYDWYN (SEQ ID NO: 8), HSFDYDWYN (SEQ ID NO: 9), HSFDYDWYNV (SEQ ID NO: 10), VHSFDYDWYNV (SEQ ID NO: 11), VHSFDYDWYNVS (SEQ ID NO: 12), SVHSFDYDWYNVS (SEQ ID NO: 13), SVHSFDYDWYNVSD (SEQ ID NO: 14), KSVHSFDYDWYNVSD (SEQ ID NO: 15), KSVHSFDYDWYNVSDQ (SEQ ID NO: 16), NKSVHSFDYDWYNVSDQ (SEQ ID NO: 17), NKSVHSFDYDWYNVSDQA (SEQ ID NO: 18), QNKSVHSFDYDWYNVSDQA (SEQ ID NO: 19), QNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 20), SQNKSVHSFDYDWYNVSDQAD (SEQ ID NO: 21), SQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 22), FSQNKSVHSFDYDWYNVSDQADL (SEQ ID NO: 23), FSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 24), SFSQNKSVHSFDYDWYNVSDQADLK (SEQ ID NO: 25), SFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 3), CSFSQNKSVHSFDYDWYNVSDQADLKN (SEQ ID NO: 26) and CSFSQNKSVHSFDYDWYNVSDQADLKNC (SEQ ID NO: 1).

4. The method of claim 3 , further comprising said single cell organism in a biofilm.

5. The method of claim 3 , wherein said single cell organism is selected from the group consisting of a bacteria, a fungus, a protozoa and an archaea.

6. The method of claim 5 , wherein said fungus comprises a yeast.

7. The method of claim 3 , wherein said surface is selected from the group consisting of a fabric, a fiber, a foam, a film, a concrete, a masonry, a glass, a metal and a plastic.

8. The method of claim 3 , wherein the peptide is from a vertebrate or an invertebrate animal.

9. The method of claim 8 , wherein the vertebrate animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

10. The method of claim 9 , wherein said mammal is a human.

11. A method of treating water to reduce biofilm formation or fouling of a filter, comprising treating the water with a peptide of claim 1 or composition of claim 2 .

12. A method of reducing biofilm formation in a fluid medium, comprising treating the fluid medium with a peptide of claim 1 or composition of claim 2 .

13. The method of claim 11 , further comprising applying said treated water or fluid medium to a reverse osmosis filter.

14. The composition of claim 2 , wherein the peptide is from a vertebrate or an invertebrate animal.

15. The composition of claim 14 , wherein the vertebrate animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

16. The composition of claim 15 , wherein the mammal is a human.

17. The composition of claim 2 , wherein the peptide is synthetic.

18. The method of claim 3 , wherein the peptide is from a vertebrate or an invertebrate animal.

19. The method of claim 18 , wherein the vertebrate animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

20. The method of claim 19 , wherein the mammal is a human.

21. The method of claim 3 , wherein the peptide is synthetic.

22. The method of claim 11 , wherein the peptide is from a vertebrate or an invertebrate animal.

23. The method of claim 22 , wherein the vertebrate animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

24. The method of claim 23 , wherein the mammal is a human.

25. The method of claim 11 , wherein the peptide is synthetic.

26. The method of claim 12 , wherein the peptide is from a vertebrate or an invertebrate animal.

27. The method of claim 26 , wherein the vertebrate animal is selected from the group consisting of a fish, an amphibian, a bird, a reptile and a mammal.

28. The method of claim 27 , wherein the mammal is a human.

29. The method of claim 12 , wherein the peptide is synthetic.

30. The method of claim 3 , wherein the composition is attached to the surface.

31. The method of claim 30 , wherein the composition is attached to the surface as a monolayer or in multiple layers.

32. The method of claim 30 , further comprising said surface in a medical device.

33. The method of claim 3 , further comprising said surface in a medical device.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 1, 2021
From: TEL HASHOMER MEDICAL RESEARCH INFRASTRUCTURE AND SERVICES LTD.
To: ZLOTKIN, AMIR
Reel/Frame 055801/0052 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 1, 2021
From: ZLOTKIN, AMIR
To: DISPERSEBIO LTD.
Reel/Frame 055801/0086 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 12, 2011
From: ZLOTKIN, AMIR
To: TEL HASHOMER MEDICAL RESEARCH, INFRASTRUCTURE AND SERVICES LTD.
Reel/Frame 026579/0912 →
Continuity (2)
Provisional Application 61193821 · Dec 29, 2008
Related Publication 20120015870A1 · Jan 19, 2012