Culture medium for epithelial stem cells and organoids comprising the stem cells
View Patent ↗The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.
1. An in vitro method for obtaining a crypt villus organoid or a colon crypt organoid, the method comprising:
incubating an epithelial stem cell or an isolated tissue fragment comprising an epithelial stem cell with an extracellular matrix, and
culturing the stem cell or isolated tissue fragment for a length of time sufficient to be able to observe formation of a crypt villus organoid or a colon crypt organoid, in an animal or human cell culture medium comprising:
a Bone Morphogenetic Protein (BMP) inhibitor,
between 5 and 500 ngram/ml of a mitogenic growth factor, and
a Wnt agonist, and
obtaining from the culture medium a crypt villus organoid or a colon crypt organoid.
2. The method according to claim 1 , wherein the BMP inhibitor is Noggin, the mitogenic growth factor is Epidermal Growth Factor, and the Wnt agonist comprises any one of R-spondin 1 through R-spondin 4.
3. The method according to claim 2 , wherein the Wnt agonist comprises any one of R-spondin 1 through R-spondin 4 and Wnt-3a.
4. The method according to claim 2 , wherein the cell culture medium further comprises a Rock (Rho-kinase) inhibitor selected from the group consisting of Y-27632, Fasudil, and H-1152.
5. The method according to claim 2 , wherein the cell culture medium further comprises a notch agonist.
6. The method according to claim 2 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
7. The method according to claim 1 , wherein the BMP inhibitor is selected from the group consisting of Noggin, DAN, Cerberus and Gremlin.
8. The method according to claim 7 , wherein the Wnt agonist is selected from the group consisting of one or more of Wnt, R-spondin 1 through R-spondin 4, Norrin, and a GSK-inhibitor.
9. The method according to claim 7 , wherein the Wnt agonist comprises any one of R-spondin 1 through R-spondin 4 and Wnt-3a.
10. The method according to claim 7 , wherein the mitogenic growth factor is EGF.
11. The method according to claim 7 , wherein the cell culture medium further comprises a Rock (Rho-kinase) inhibitor selected from the group consisting of Y-27632, Fasudil, and H-1152.
12. The method according to claim 7 , wherein the cell culture medium further comprises a notch agonist.
13. The method according to claim 7 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
14. The method according to claim 1 , wherein the Wnt agonist is selected from the group consisting of one or more of Wnt, R-spondin 1 through R-spondin 4, Norrin, and a GSK-inhibitor.
15. The method according to claim 14 , wherein the Wnt agonist comprises any one of R-spondin 1 through R-spondin 4 and Wnt-3a.
16. The method according to claim 14 , wherein the mitogenic growth factor is EGF.
17. The method according to claim 14 , wherein the cell culture medium further comprises a Rock (Rho-kinase) inhibitor selected from the group consisting of Y-27632, Fasudil, and H-1152.
18. The method according to claim 14 , wherein the cell culture medium further comprises a notch agonist.
19. The method according to claim 14 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
20. The method according to claim 1 , wherein the Wnt agonist is one of R-spondin 1 through R-spondin 4 and Wnt-3a.
21. The method according to claim 20 , wherein the cell culture medium further comprises a Rock (Rho-kinase) inhibitor selected from the group consisting of Y-27632, Fasudil, and H-1152.
22. The method according to claim 20 , wherein the cell culture medium further comprises a notch agonist.
23. The method according to claim 20 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
24. The method of claim 1 , wherein the cell culture medium further comprises a Rock (Rho-kinase) inhibitor selected from the group consisting of Y-27632, Fasudil, and H-1152.
25. The method according to claim 24 , wherein the cell culture medium further comprises a notch agonist.
26. The method according to claim 24 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
27. The method according to claim 1 , wherein the cell culture medium further comprises a notch agonist.
28. The method according to claim 27 , wherein the cell culture medium further comprises brain-derived neurotrophic factor.
29. The method according to claim 1 , wherein the mitogenic growth factor is Epidermal Growth Factor.
30. The method according to claim 1 , wherein the epithelial stem cell is an adenoma cell.
31. An in vitro method for obtaining and/or culturing a colon crypt, the method comprising:
culturing epithelial stem cells or isolated tissue fragments comprising epithelial stem cells in contact with an extracellular matrix for a length of time sufficient to be able to observe formation of a colon crypt, in a medium comprising Noggin, EGF, any one of R-spondin 1 through R-spondin 4 and/or Wnt3, B27, N2, and N-Acetylcysteine; and
obtaining from the culture medium a colon crypt.
32. The method according to claim 31 , wherein the epithelial stem cells are adenoma cells.
33. An in vitro method for obtaining a crypt villus organoid or colon crypt organoid, the method comprising:
culturing epithelial stem cells or isolated tissue fragments comprising epithelial stem cells on an extracellular matrix for a length of time sufficient to be able to observe formation of a crypt villus organoid or a colon crypt organoid, in an animal or human cell culture medium comprising:
a Bone Morphogenetic Protein (BMP) inhibitor;
a Wnt agonist; and
between 5 and 500 nanogram/ml of Epidermal Growth Factor (EGF); and
obtaining from the culture medium a crypt villus organoid or a colon crypt organoid.
34. The method according to claim 33 , wherein the epithelial stem cells are adenoma cells.
35. An in vitro method for obtaining a crypt villus organoid or a colon crypt organoid the method comprising:
incubating an epithelial stem cell or an isolated tissue fragment comprising an epithelial stem cell with an extracellular matrix, wherein the epithelial stem cell is an adenoma cell, and
culturing the stem cell or isolated tissue fragment for a length of time sufficient to be able to observe formation of a crypt villus organoid or a colon crypt organoid, in an animal or human cell culture medium comprising:
a Bone Morphogenetic Protein (BMP) inhibitor, and
between 5 and 500 ngram/ml of a mitogenic growth factor, and
obtaining from the culture medium a crypt villus organoid or a colon crypt organoid.