Method for analyzing PSA and method for distinguishing prostate cancer from prostatic hypertrophy using that method for analyzing PSA
A method for distinguishing prostate cancer from prostatic hypertrophy using the method for analyzing PSA and an analysis kit of PSA are provided. An object of the present invention can be solved by being brought into contact a lectin having an affinity for p-N-acetylgalactosamine residues and/or a lectin having an affinity for fucose a(I, 2) galactose residues with a sample possibly containing PSA, to determine an amount of PSA having an affinity for the lectin. A method for distinguishing prostate cancer from prostatic hypertrophy can be provided by this method.
1. A method for analyzing PSA with a β-N-acetylgalactosamine residue, comprising the following step of:
bringing into contact Wisteria floribunda agglutinin with a sample, which sample is a member selected from the group consisting of blood, serum, and plasma possibly containing PSA, wherein the amount of PSA is determined by measuring total PSA or free PSA, to determine an amount of PSA having an affinity for Wisteria floribunda agglutinin.
2. The method for analyzing PSA with a β-N-acetylgalactosamine residue according to claim 1 , wherein the amount of PSA having an affinity for Wisteria floribunda agglutinin is determined
(1) by measuring an amount of separated PSA having an affinity for Wisteria floribunda agglutinin,
(2) by measuring an amount of PSA in a sample before the separation and an amount of the separated PSA having an affinity for Wisteria floribunda agglutinin, or
(3) by measuring an amount of PSA in a sample, which sample is a member selected from the group consisting of blood, serum and plasma before the separation and an amount of the separated PSA lacking an affinity for Wisteria floribunda agglutinin.
3. The method for analyzing PSA with a β-N-acetylgalactosamine residue according to claim 1 , wherein the sample, which sample is a member selected from the group consisting of blood, serum, and plasma, is obtained from a patient suspected of having prostate cancer.
4. A method for analyzing PSA having a β-N acetylgalactosamine residue, the method comprising:
contacting a sample with Wisteria floribunda agglutinin, wherein the sample is a member selected from the group consisting of blood, serum, and plasma possibly containing PSA; and
determining an amount of PSA having an affinity for Wisteria floribunda agglutinin, wherein the amount of PSA having an affinity for Wisteria floribunda agglutinin is greater for PSA associated with prostate cancer than non-prostate cancer PSA.