Method for preparing biological tissues for use in biological prostheses
View Patent ↗A method of treating a biological tissue for biological prostheses includes steps of fixation of the biological tissue via a fixing solution including glutaraldehyde and detoxification of the fixed biological tissue via treatment with a detoxifying solution. The detoxification step includes one or both of eliminating phospholipids via treatment with an elimination solution and a treatment with a detoxifying solution. The elimination solution includes 1,2-octanediol and ethanol. The detoxifying solution includes taurine or homocysteic acid.
1. A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
eliminating phospholipids from the biological tissue via treatment with an elimination solution including 5% v/v 1,2-octanediol and 40% v/v ethanol at a pH of 7.2 and a temperature of 37° C.;
fixing the biological tissue via a treatment with a fixing solution containing 0.5% v/v glutaraldehyde in phosphate buffer at a pH of 7.4; and
detoxifying the fixed biological tissue via treatment with a detoxifying solution including taurine or homocysteic acid in phosphate buffer at pH 7 and at a temperature above room temperature.
2. The method according to claim 1 , wherein detoxification occurs at a temperature above 30° C.
3. The method according to claim 1 , wherein elimination of phospholipids is carried out for a time period in the range of 2 to 7 days.
4. The method according to claim 1 , wherein elimination of phospholipids is carried out for a time period in the range of 16 to 72 hours.
5. The method according to claim 1 , wherein the detoxifying solution includes taurine at a concentration expressed as w/v in the range from 0.10% to saturation.
6. The method according to claim 1 , wherein the detoxifying solution includes taurine at a concentration expressed as w/v in the range of 0.20% to 1.00%.
7. The method according to claim 1 , wherein the detoxifying solution includes homocysteic acid at a concentration expressed as w/v in the range from 0.10% to saturation.
8. The method according to claim 1 , wherein the detoxifying solution includes homocysteic acid at a concentration expressed as w/v in the range of 0.50% to 5.00%.
9. The method according to claim 1 , wherein the detoxification step is carried out at a temperature greater than 30° C.
10. The method according to claim 1 , wherein the detoxification step is carried out at a temperature between 35° C. and 45° C.
11. The method according to claim 1 , wherein the detoxification step is carried out for a period in the range of 2 to 36 hours.
12. The method according to claim 1 , wherein the detoxification step is carried out for a period of 12 to 48 hours.