Method involving 1-benzotriazolyl carbonate esters of polymers
View Patent ↗The invention provides a method comprising the steps of (i) reacting a water-soluble and non-peptidic polymer having two or more terminal hydroxyl groups with di(1-benzotriazolyl)carbonate to form a water-soluble and non-peptidic polymer having two or more 1-benzotriazolylcarbonate ester groups; and (ii) reacting the water-soluble and non-peptidic polymer having two or more 1-benzotriazolylcarbonate ester groups with a water-soluble and non-peptidic polymer having three or more primary amino groups under conditions effective to form a cross-linked polymer composition.
1. A method comprising reacting PEG-BTC esters with lysine.
2. The method of claim 1 , wherein the PEG-BTC esters have the following structure:
wherein R′ is a capping group.
3. The method of claim 2 , wherein the capping group is methoxy.
4. The method of claim 1 , wherein the PEG has an average molecular weight from about 200 Da to about 100,000 Da.
5. The method of claim 1 , resulting in the formation of a polymeric lysine derivative having the following structure:
wherein PEG is a poly(ethylene glycol) and Z is selected from the group consisting of H, N-succinimidyl, and 1-benzotriazolyl.
6. The method of claim 5 , wherein each PEG is capped with a capping group.
7. The method of claim 6 , wherein the capping group is methoxy.