METHOD OF PREDICTING CLINICAL OUTCOMES FOR MELANOMA PATIENTS USING CIRCULATING MELANOMA CELLS IN BLOOD
The present invention provides an automated method for capturing and detecting circulating melanoma cells (CMC's) in the blood of patients with melanoma. The absolute number of circulating melanoma cells detected in the peripheral blood tumor load is, in part, a factor in prediction of survival, time to progression, and response to therapy.
1 . A method of predicting overall survival for patients with metastatic melanoma comprising:
(a) obtaining a 7.5 mL blood sample from a patient with metastatic melanoma, said sample comprising a mixed cell population suspected of containing circulating melanoma cells;
(b) enriching a fraction of said specimen, said fraction containing said circulating melanoma cells;
(c) confirming structural integrity of said rare cells to be intact;
(d) analyzing said intact rare cells; wherein said analyzing correlates disease progression;
(e) evaluating the number of circulating melanoma cells in said blood sample
wherein if the number is greater than or equal to 2 predicting that the patient's overall survival will be low, and
wherein if the number of circulating melanoma cells is less than two, predicting that the patient's overall survival will be high.
2 . A method as claimed in claim 1 , wherein said fraction is obtained by immunomagnetic enrichment using an externally applied magnetic field to separate paramagnetic particles coupled to a biospecific ligand which specifically binds to said melanoma cells, to the substantial exclusion of other populations.
3 . A method as claimed in claim 2 , wherein said biospecific ligand is melanoma cell adhesion molecule CD146.
4 . A method as claimed in claim 1 , wherein said structural integrity is determined by a procedure selected from the group consisting of immunocytochemical procedures, FISH procedures, flowcytometry procedures, image cytometry procedures, and combinations thereof.
5 . A method as claimed in claim 1 , wherein said structural integrity is determined by a nucleic acid dye, a monoclonal antibody specific for High Molecular Weight Melanoma Associated Antigen.
6 . The method as claimed in claim 5 , wherein said structural integrity is further confirmed by exclusion of co-enriched leukocytes and circulating endothelial cells using leukocyte and endothelial specific antibodies.
7 . The method of claim 6 , wherein said specific antibodies are CD45 and CD34.
8 . The method as claimed in claim 5 further containing CD45 and CD34 to exclude co-enriched leukocytes and circulating endothelial cells.
9 . The method as claimed in claim 1 , wherein FITC labeled anti-Ki67 is added to determine the proportion of CMC's in active cell cycle within the circulation.
10 . The method of claim 1 wherein low overall survival is no more than two months.
11 . The method of claim 1 wherein high overall survival is twelve months