Kit for detecting HIV-2 and method for detecting HIV-2 using the same
A kit for detecting HIV-2 strains in a test sample is disclosed. In addition a method is described for the real-time detection of HIV-2 strains in a test sample using the kit. According to method of detection, the results of the detection can be rapidly identified with a reduced number of copies of a sample in real-time.
1. A kit for detecting HIV-2 strains, said kit comprising a pair of primers and a probe selected from the group consisting of the following primer sets and probes:
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 1 and a primer having the nucleotide sequence of SEQ ID NO: 9 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 2 and a primer having the nucleotide sequence of ID NO: 6 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 2 and a primer having the nucleotide sequence of SEQ ID NO: 7 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 2 and a primer having the nucleotide sequence of SEQ ID NO: 8 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 2 and a primer having the nucleotide sequence of SEQ ID NO: 9 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 3 and a primer having the nucleotide sequence of SEQ ID NO: 8 and a probe having the nucleotide sequence of SEQ ID NO: 10;
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 4 and a primer having the nucleotide sequence of SEQ ID NO: 8 and a probe having the nucleotide sequence of SEQ ID NO: 10
a primer set comprising a primer having the nucleotide sequence of SEQ ID NO: 5 and a primer having the nucleotide sequence of SEQ ID NO: 8 and a probe having the nucleotide sequence of SEQ ID NO: 10, and
a primer set comprising a first primer having the nucleotide sequence of SEQ ID NO: 4, a second primer having the nucleotide sequence of SEQ ID NO: 5, and a third primer having the nucleotide sequence of SEQ ID NO: 8 and a probe having the nucleotide sequence of SEQ ID NO: 10.
2. The kit of claim 1 , which further comprises an amplifying polymerase activity and an RNase H activity.
3. The kit of claim 1 , which further comprises a reverse transcriptase activity.
4. The kit of claim 1 , wherein a 5′ end of each probe is labeled with a fluorescence label, or a 3′ end of each of the probes is labeled with a fluorescence quencher.
5. The kit of claim 1 , further comprising a mixture comprising dATP, dCTP, dGTP, and dTTP; a DNA polymerase; RNase HII; and a buffer solution.
6. The kit of claim 1 , further comprising uracil-N-glycosylase.
7. The kit of claim 1 , wherein the probe is linked to a solid support.
8. The kit of claim 1 , wherein the probe is present as a free form in a solution.
9. The kit of claim 2 , wherein the amplifying polymerase activity is the activity of a thermostable DNA polymerase.
10. The kit of claim 2 , wherein the RNAse H activity is the activity of a thermostable RNAse H.
11. The kit of claim 2 , wherein the RNAse H activity is a hot start RNAse H activity.