Generation of induced pluripotent stem cells from small volumes of peripheral blood
Methods and compositions relating to the production of induced pluripotent stem cells (iPS cells) are disclosed. For example, induced pluripotent stem cells may be generated from peripheral blood cells, such as human blood progenitor cells, using episomal reprogramming and feeder-free or xeno-free conditions. In certain embodiments, the invention provides novel methods for improving overall reprogramming efficiency with low number of blood progenitor cells.
1. A method for producing human iPS cells from hematopoietic progenitor cells, the method comprising the steps of:
a) providing a cell population of non-mobilized human peripheral blood cells comprising hematopoietic progenitor cells;
b) culturing said population under expansion conditions in a defined culture to promote the expansion of said hematopoietic progenitor cells;
c) introducing exogenous episomal genetic elements comprising vectors that comprise an OriP replication origin, a nucleotide sequence encoding EBNA-1 or a derivative thereof which has a deletion of residues corresponding to residues 65 to 89 of EBNA-1, and/or a deletion of residues corresponding to residues 90 to 328 of EBNA-1, and one or more expression cassettes encoding iPS reprogramming factors into said expanded hematopoietic progenitor cells, wherein the exogenous episomal genetic elements have one or more polycistronic cassettes; and
d) culturing said expanded hematopoietic progenitor cells under defined culture conditions, thereby producing human iPS cells from said hematopoietic progenitor cells.
2. The method of claim 1 , wherein the cell population is comprised in a blood sample having up to about 10 ml in volume.
3. The method of claim 1 , wherein the expansion conditions comprise an expansion medium comprising one or more cytokines including stem cell factor (SCF), Flt-3 ligand (Flt3L), thrombopoietin (TPO), Interleukin 3 (IL-3), or Interleukin 6 (IL-6).
4. The method of claim 3 , wherein the expansion conditions do not comprise a Notch-1 ligand.
5. The method of claim 1 , wherein the expansion conditions in step b) comprise a defined extracellular matrix.
6. The method of claim 1 , wherein the expansion conditions in step b) does not comprise a matrix.
7. The method of claim 1 , wherein the expansion conditions in step b) have or the culture in step d) has up to 7% oxygen tension.
8. The method of claim 1 , wherein the reprogramming factors are Sox, Oct, Nanog, Lin-28, Klf4, C-myc (or L-myc), SV40 large T-antigen, or a combination thereof.
9. The method of claim 1 , wherein the step c) occurs at about days 3, 4, 5 or 6 of the expansion step b).
10. The method of claim 1 , wherein the starting number of the expanded hematopoietic progenitor cells in the step c) is from about 10 4 to about 10 5 .
11. The method of claim 1 , wherein the culture in step d) comprises a defined extracellular matrix.
12. The method of claim 11 , wherein the defined extracellular matrix has a single type of extracellular matrix peptide.
13. The method of claim 12 , wherein the defined extracellular matrix is a human fibronectin fragment.
14. The method of claim 1 , further comprising: e) selecting for the iPS cells.
15. A method for producing human iPS cells from a peripheral blood sample, the method comprising the steps of:
a) providing a peripheral blood sample comprising hematopoietic progenitor cells, wherein the peripheral blood sample has a volume of up to 50 ml, wherein the cell population is from one or more subjects whose cells have not been mobilized to expand CD34+ cells;
b) culturing said hematopoietic progenitor cells under expansion conditions in a defined culture to promote the expansion of said hematopoietic progenitor cells;
c) introducing exogenous episomal genetic elements comprising vectors that comprise an OriP replication origin, a nucleotide sequence encoding EBNA-1 or a derivative thereof which has a deletion of residues corresponding to residues 65 to 89 of EBNA-1, and/or a deletion of residues corresponding to residues 90 to 328 of EBNA-1, and one or more expression cassettes encoding iPS reprogramming factors into said expanded hematopoietic progenitor cells; and
d) culturing said hematopoietic progenitor cells under defined culture conditions, thereby producing human iPS cells from said peripheral blood sample.
16. A cell culture composition comprising a cell population of human peripheral blood cells comprising hematopoietic progenitor cells and progeny cells thereof, a defined extracellular matrix, and a defined medium, wherein the hematopoietic progenitor cells comprise one or more exogenous episomal genetic elements comprising polycistronic vectors that comprise an OriP replication origin, a nucleotide sequence encoding EBNA-1 or a derivative thereof which has a deletion of residues corresponding to residues 65 to 89 of EBNA-1, and/or a deletion of residues corresponding to residues 90 to 328 of EBNA-1, and one or more expression cassettes encoding iPS cell reprogramming factors.
17. The method of claim 15 , wherein the peripheral blood sample has a volume of up to 20 ml.
18. The method of claim 15 , wherein the peripheral blood sample has a volume of up to 15 ml.
19. The method of claim 15 , wherein the peripheral blood sample has a volume of up to 10 ml.