MicroRNA polymorphisms conferring enhanced drought tolerance in a plant
View Patent ↗Methods of identifying a single nucleotide polymorphism associated with a plant trait and methods of identifying a plant having an improved trait. The plant trait is correlated with at least one single nucleotide polymorphism in a microRNA region of a plant genome. Isolated nucleic acids, transgenic plants, and methods of producing the same are also disclosed.
1. A method of producing a maize plant using marker-assisted breeding, wherein said maize plant confers increased grain yield at standard moisture percentage, the method comprising the steps of:
(a) crossing a first maize plant or a progeny thereof with a second maize plant, wherein said first maize plant or progeny thereof has been selected for said crossing based on the presence of at least one polymorphism within a marker locus of its genome, wherein said marker locus is associated with increased grain yield at standard moisture percentage and wherein said marker locus is SEQ ID NO: 45;
(b) producing a progeny plant population from the cross of (a);
(c) selecting a progeny plant from the progeny plant population of (b) based on genotyping the progeny plant's genomic DNA and selecting a progeny plant having at least one polymorphism within the marker locus of its genome, wherein said marker locus is associated with increased grain yield at standard moisture percentage and wherein said marker locus is SEQ ID NO: 45; and
(d) producing a maize plant using marker-assisted breeding wherein said maize plant exhibits increased grain yield at standard moisture percentage.
2. The method of claim 1 , wherein the single nucleotide polymorphism of steps (a) and (c) is located at a nucleotide corresponding to a position selected from the group consisting of: (a) position 216 of SEQ ID NO: 45, wherein the nucleotide is a C; (b) position 301 of SEQ ID NO: 45, wherein the nucleotide is a C; and (c) position 743 of SEQ ID NO: 45, wherein the nucleotide is a T.
3. The method of claim 1 , wherein the maize plant produced in (d) further exhibits any one of the following traits selected from the group consisting of: decreased grain moisture adjusted percentage, decreased grain moisture at harvest, increased grain yield at harvest moisture percentage, and increased ear height in cm.