Chloral hydrate detection
View Patent ↗The invention relates to an immunoassay method and kit for the indirect detection of chloral hydrate. The invention is underpinned by a novel immunogen that produces an antibody that is specific for the chloral hydrate metabolite trichloroethanol glucuronide. Detection and quantification of trichloroethanol glucuronide has important applications in clinical toxicology, drug facilitated crime, water testing and solvent exposure.
1. A method of detecting and/or quantifying trichloroethanol glucuronide (TCG) in an individual or a solution, the method comprising contacting an in vitro sample taken from the individual or a solution with a conjugate and an antibody that specifically binds to TCG, wherein said antibody generated against an immunogen of the structure
where n=0 or 1; and where, when n=1, the crosslinker joins the carbonyl substituent of the tetrahydropyran ring to an antigenicity conferring carrier material (accm), and said antibody has less than 0.69% cross-reactivity to chloral hydrate, trichloroacetic acid and ethyl glucuronide as compared to reactivity of TCG, detecting the bound conjugate, and deducing from a calibrator value or calibrator values the presence or amount of TCG.
2. The method of claim 1 in which the in vitro sample is urine.
3. The method of claim 1 wherein said antibody is generated against an immunogen of the structure
in which n=1 and the crosslinker is —X—Y—Z—,
where X is a heteroatom selected from nitrogen, oxygen or sulphur;
Y is a C 1 -C 10 substituted or unsubstituted straight chain alkylene moiety, or arylene moiety; and
Z, before conjugation with the accm, is selected from a carboxy, a dithiopyridyl, a maleimide, an amino, a hydroxyl, a thiol, a thioester or an aldehyde moiety.
4. The method of claim 1 wherein said antibody is generated against an immunogen of the structure
in which n=0 and the accm is selected from bovine serum albumin, egg ovalbumin, bovine gamma globulin, bovine thyroglobulin or keyhole limpet haemocyanin.
5. The method of claim 1 wherein said antibody is generated against an immunogen of the structure
in which n=0 and the accm is bovine thyroglobulin.