Maleamic acid polymer derivatives and their bioconjugates
View Patent ↗The present invention is directed a method comprising administering a composition to an individual, wherein the composition comprises a plurality of conjugates, each conjugate in the plurality a protein derivatized with a water-soluble polymer, wherein the polymer is coupled to the protein via succinimide groups covalently attached to either cysteine sulfhydryl groups or lysine amino groups, and substantially all of the succinimide groups present in the composition are present in a ring-opened form.
1. A method comprising administering a composition to an individual, wherein the composition comprises a plurality of conjugates, each conjugate in the plurality a protein derivatized with a water-soluble polymer, wherein the polymer is coupled to the protein via succinimide groups covalently attached to either cysteine sulfhydryl groups or lysine amino groups, and substantially all of the succinimide groups present in the composition are present in a ring-opened form.
2. The method of claim 1 , wherein 95% or greater of all the succinimide groups present in the composition are present in a ring-opened form.
3. The method of claim 1 , wherein the polymer is coupled to the protein via succinimide groups covalently attached to cysteine sulfhydryl groups.
4. The method of claim 3 , wherein 95% or greater of all the succinimide groups present in the composition are present in a ring-opened form.
5. The method of claim 1 , wherein the polymer is coupled to the protein via succinimide groups covalently attached to lysine amino groups.
6. The method of claim 5 , wherein 95% or greater of all the succinimide groups present in the composition are present in a ring-opened form.
7. The method of claim 1 , wherein the water-soluble polymer is selected from the group consisting of a poly(alkylene oxide), poly(vinyl pyrrolidone), poly(vinyl alcohol), polyoxazoline, poly(acryloylmorpholine), and poly(oxyethylated polyol).
8. The method of claim 7 , wherein the water-soluble polymer is a poly(alkylene oxide).
9. The method of claim 8 , wherein the water-soluble polymer is a poly(ethylene glycol).
10. The method of claim 9 , wherein said poly(ethylene glycol) comprises an end-capping moiety.
11. The method of claim 10 , wherein said end-capping moiety is selected from the group consisting alkoxy, substituted alkoxy, alkenyloxy, substituted alkenyloxy, alkynyloxy, substituted alkynyloxy, aryloxy, and substituted aryloxy.
12. The method of claim 11 , wherein said end-capping moiety is selected from the group consisting of methoxy, ethoxy, and benzyloxy.
13. The method of claim 9 , wherein said poly(ethylene glycol) has a nominal average molecular mass of from about 100 daltons to about 100,000 daltons.
14. The method of claim 13 , wherein said poly(ethylene glycol) has a nominal average molecular mass of from about 1,000 daltons to about 80,000 daltons.
15. The method of claim 14 , wherein said poly(ethylene glycol) has a nominal average molecular mass of from about 2,000 daltons to about 50,000 daltons.
16. The method of claim 9 , wherein said poly(ethylene glycol) has a structure selected from the group consisting of linear, branched, forked, and multi-armed.
17. The method of claim 1 , wherein said water-soluble polymer comprises a linker, L, interposed between said water-soluble polymer and said maleimide group.
18. The method of claim 9 , wherein said poly(ethylene glycol) is directly attached to the nitrogen atom of said maleimide group.