Nucleic acid construct having an ovalbumin promotor
View Patent ↗A transgenic avian containing in its genome an exogenous nucleotide sequence which includes a promoter component and a vector with reduced promoter interference wherein the exogenous nucleotide sequence is integrated into the genome and the avian.
1. An isolated nucleic acid molecule at least 95% identical to a nucleic acid molecule comprising:
a 5′ LTR as set forth in SEQ ID NO:29;
an ovalbumin promoter fragment comprising DHS I & II as set forth in the nucleotide sequence 5209 through 8311 of SEQ ID NO:22;
reverse responsive element as set forth in SEQ ID NO:25;
constitutive transport element as set forth in SEQ ID NO:26
a 3′ UTR as set forth in the nucleotide sequence 13576 through 15163 of SEQ ID NO:22.
2. An isolated vector comprising the nucleic acid molecule of claim 1 .
3. The vector of claim 2 , wherein the vector is a self-inactivating vector derived from a retrovirus.
4. The vector of claim 3 , wherein the retrovirus is selected from the group consisting of an avian leukosis virus (ALV), a murine leukemia virus (MLV), a moloney murine leukemia virus (MMLV) and a lentivirus.
5. The vector of claim 4 , wherein the retrovirus is an avian leukosis virus.
6. The vector of claim 5 , wherein the self-inactivation vector has no selectable marker.
7. An isolated host cell containing the vector of claim 2 .