Composition for preserving platelets during photosensitization
A platelet preservation composition is disclosed. The composition comprises a photosensitizer, an inhibitor of platelet activation, and/or an anticoagulant. The preservation composition allows inactivation of pathogens in a platelet preparation while maintaining the functionality of the platelets.
1. A preserved platelet composition, comprising:
platelets;
an effective amount of a photosensitizer for pathogen inactivation;
a platelet activation inhibitor; and
an anticoagulant,
wherein the platelet activation inhibitor is selected from the group consisting of eptifibatide, tirofiban, abciximab, lefradafiban, sibrafiban, orbofiban, xemilofiban, lotrafiban, XJ757 and XR299, wherein the anticoagulant is a direct inhibitor of factor Xa or factor IIa,
wherein the preserved platelet composition is sterilized by exposure to a radiation at a wavelength that sensitizes the photosensitizer, and wherein the platelet composition is substantially free of red blood cells.
2. The platelet preservation composition of claim 1 , wherein the photosensitizer comprises riboflavin, psoralen or methylene blue.
3. The composition of claim 1 , wherein the anticoagulant is selected from the group consisting of DX-9065a, RPR-120844, BX-807834, SEL-1915, SEL-2219, SEL-2489 and SEL-2711.
4. The composition of claim 1 , wherein the anticoagulant is selected from the group consisting of DUP714, hirulog and melgatran.
5. The composition of claim 1 , further comprising a hemoglobin-based oxygen carrier.