Instruments and methods for mixing the contents of a detection chamber
View Patent ↗A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
1. An instrument for processing a sample in a receptacle having a plurality of interconnected chambers, the instrument comprising:
a receptacle-receiving area for receiving the receptacle;
a detector having components positioned to detect a light signal from the contents of a detection chamber of the receptacle when the receptacle is placed in an operative position in said receptacle-receiving area, said components comprising a light source and a signal detecting element disposed on the same side of the receptacle-receiving area;
at least one movable compression element disposed to be adjacent the detection chamber and situated between the receptacle and said light source and said signal detecting element of said detector when the receptacle is placed in the operative position in said receptacle-receiving area, wherein said compression element is configured to move relative to the detector and to transmit light from said light source to said detection chamber and to transmit light signals from said detection chamber to said signal detecting element;
an actuator constructed and arranged to move the compression element; and
a controller programmed to activate the actuator to move said compression element into engagement with a flexible portion of the detection chamber to thereby depress the flexible portion to agitate the contents of the detection chamber or to move the contents of the detection chamber into an adjacently connected chamber of the receptacle.
2. The instrument of claim 1 , wherein the actuator is a pneumatic actuator.
3. The instrument of claim 1 , wherein the compression element includes a window that permits the light signal to pass to the lens of the detector.
4. The instrument of claim 1 , wherein the detector is fixed in the instrument.
5. The instrument of claim 1 , wherein the compression element has a tubular body in sliding engagement with a light-directing conduit of the detector.
6. The instrument of claim 1 , wherein an end surface of the compression element generally conforms to the shape of the detection chamber.
7. The instrument of claim 1 , wherein the compression element is one of a plurality of compression elements arranged to engage a plurality of the chambers of the receptacle.
8. The instrument of claim 1 , wherein the compression element has a tubular body in sliding engagement with a light-directing conduit of the detector, the receptacle is carried adjacent to an actuator plate disposed between the receptacle and the detector, and the compression element is in alignment with an opening formed through the actuator plate with the detection chamber disposed adjacent the opening, and wherein the instrument further includes:
a bracket extending from the actuator plate;
an actuator carried on the bracket and controlled by the controller for selective reciprocal movement; and
an actuating bar extending from the compression element to the actuator and adapted to translate movement of the actuator to the compression element such that reciprocal movement of the actuator causes corresponding reciprocal movement of the compression tube between a retracted position and an extended position engaged with the detection chamber.
9. The instrument of claim 8 , further comprising a guide rod extending from the actuator plate and into engagement with a portion of the actuating bar and adapted to guide the compression tube during movement of the compression tube.
10. The instrument of claim 8 , wherein the compression element includes a window that permits the light signal to pass to the light-directing conduit of the detector.
11. The instrument of claim 8 , wherein the actuator comprises a pneumatic piston.
12. The instrument of claim 1 , further comprising a thermal element including at least a portion thereof disposed to be adjacent the detection chamber on a side of the receptacle-receiving area opposite the compression element.