Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics
View Patent ↗Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants with increased tolerance to abiotic stress (e.g., high or low temperature, drought, flood).
1. A plant, plant cell, plant material or seed of a plant which comprises a nucleic acid molecule comprising:
a) a first nucleic acid having a nucleotide sequence which encodes an amino acid sequence exhibiting at least 95% sequence identity to SEQ ID NO: 11, or a first nucleic acid having the nucleotide sequence of SEQ ID NO:10 which encodes SEQ ID NO: 11; and
(b) a second nucleic acid comprising a regulatory sequence capable of causing transcription and/or translation of said first nucleic acid, wherein said first and second nucleic acids are operably linked and heterologous to each other, and wherein said plant has improved water use efficiency characteristics as compared to a wild-type plant cultivated under the same conditions.
2. A transgenic plant having a gene construct comprising a nucleic acid encoding a water use efficiency component operably linked to a plant promoter so that the water use efficiency component is ectopically overexpressed in the transgenic plant, and the transgenic plant exhibits:
i) faster rate of growth,
ii) greater fresh or dry weight at maturation,
iii) greater fruit or seed yield,
iv) higher tolerance to heat,
v) higher tolerance to drought,
vi) higher tolerance to cold,
vii) greater germination rate,
viii) reduced water consumption, or
ix) greater tolerance to flood
than a progenitor plant which does not contain the gene construct, when the transgenic plant and the progenitor plant are cultivated under identical environmental conditions, wherein the water use efficiency component is a nucleic acid molecule encoding a polypeptide having at least 95% identity to SEQ ID NO:11, and wherein said nucleic acid encoding a water use efficiency component is heterologous to said plant promoter.
3. The plant, plant cell, plant material or seed of a plant according to claim 1 , wherein said first nucleic acid has a nucleotide sequence which encodes the amino acid sequence of SEQ ID NO: 11.
4. The plant, plant cell, plant material or seed of a plant according to claim 1 , wherein said first nucleic acid sequence has the sequence of SEQ ID NO: 10 which encodes SEQ ID NO: 11.
5. The transgenic plant according to claim 2 , wherein said nucleic acid molecule encoding said polypeptide has the amino acid sequence of SEQ ID NO: 11.
6. The transgenic plant according to claim 2 , wherein said nucleic acid molecule has the nucleotide sequence of SEQ ID NO: 10 which encodes a polypeptide having the amino acid sequence of SEQ ID NO: 11.