SYSTEMS, DEVICES, AND METHODS FOR AUTOMATED CHARACTERIZATION OF A NUCLEIC ACID MOLECULE
The invention generally relates to systems, cartridges, and methods for automated characterization of a nucleic acid molecule, in particular, optical mapping of DNA from an organism. In certain embodiments, the invention provides a cartridge for characterizing a nucleic acid molecule, the cartridge including a reaction chamber having a derivatized bottom surface, at least one reagent reservoir, and a pump, in which the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other.
1 . A cartridge for characterizing a nucleic acid molecule, the cartridge comprising:
a reaction chamber having a derivatized bottom surface;
at least one reagent reservoir; and
a pump;
wherein the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other.
2 . The cartridge according to claim 1 , wherein the cartridge is operably linked to a computerized preparation station.
3 . The cartridge according to claim 1 , wherein the at least one reagent reservoir is a plurality of reservoirs.
4 . The cartridge according to claim 3 , wherein each reservoir further comprises a loading port and a computer controlled valve for controlling flow of reagents from the reservoirs to the reaction chamber.
5 . The cartridge according to claim 4 , wherein a first reservoir holds a TE wash reagent, a second reservoir holds a buffer, a third reservoir holds an enzyme, and a fourth reservoir holds a nucleic acid stain.
6 . The cartridge according to claim 1 , further comprising a reagent waste pad.
7 . The cartridge according to claim 1 , further comprising a channel forming device configured to mate with the reaction chamber.
8 . The cartridge according to claim 1 , further comprising a reaction chamber cap.
9 . The cartridge according to claim 1 , further comprising a heater/cooling device.
10 . The cartridge according to claim 9 , wherein the heater/cooling device is located beneath the reaction chamber.
11 . The cartridge according to claim 1 , wherein the bottom surface of the reaction chamber is composed of derivatized glass.
12 . A method for characterizing a nucleic acid molecule, the method comprising:
applying one or more nucleic acid molecules to a derivatized bottom surface of a reaction chamber, wherein the nucleic acid molecules are elongated and fixed onto the surface so that the nucleic acid molecules remain accessible for enzymatic reactions;
flowing reagents to and from the reaction chamber to wash, enzymatically digest, and stain the nucleic acid molecules to obtain one or more restriction digests of the molecule; and
imaging the restriction digests, thereby characterizing the nucleic acid molecule.
13 . The method according to claim 12 , further comprising constructing an optical map from the restriction digests.
14 . The method according to claim 12 , wherein prior to said flowing step, the method further comprises applying a cap to the reaction chamber.
15 . The method according to claim 12 , wherein prior to said flowing step, the method further comprises tilting the cartridge at a 60° angle.
16 . The method according to claim 12 , wherein flowing is controlled by pumps and valves that are operably connected to a computerized preparation station.
17 . The method according to claim 12 , wherein the derivatized bottom surface of the reaction chamber is composed of derivatized glass.
18 . The method according to claim 12 , wherein the nucleic acid molecule is deoxyribonucleic acid or ribonucleic acid.
19 . A cartridge for characterizing a nucleic acid molecule, the cartridge comprising:
a reaction chamber having a derivatized bottom surface, wherein a nucleic acid molecule is elongated and fixed onto the derivatized bottom surface of the reaction chamber so that the nucleic acid molecule remains accessible for enzymatic reactions;
at least one reagent reservoir; and
a pump;
wherein the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other.
20 . A system for characterizing a nucleic acid molecule, the system comprising:
a cartridge comprising: a reaction chamber having a derivatized bottom surface, wherein a nucleic acid molecule is elongated and fixed onto the derivatized bottom surface of the reaction chamber so that the nucleic acid molecule remains accessible for enzymatic reactions; at least one reagent reservoir; and a pump; wherein the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other; and
a computerized preparation station operably linked to the cartridge, wherein the station controls flow of reagents to and from the reaction and chamber.