Microorganisms and methods for the biosynthesis of aromatics, 2,4-pentadienoate and 1,3-butadiene
View Patent ↗The invention provides non-naturally occurring microbial organisms having a toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene pathway. The invention additionally provides methods of using such organisms to produce toluene, benzene, p-toluate, terephthalate, (2-hydroxy-3-methyl-4-oxobutoxy)phosphonate, (2-hydroxy-4-oxobutoxy)phosphonate, benzoate, styrene, 2,4-pentadienoate, 3-butene-1ol or 1,3-butadiene.
1. A non-naturally occurring microbial organism having a 1,3-butadiene pathway, wherein said microbial organism comprises the following set of said 1,3-butadiene pathway enzymes (i) acrylyl-CoA acetyltransferase, (ii) 3-oxopent-4-enoyl-CoA reductase, (iii) 3-hydroxypent-4-enoyl-CoA transferase, synthetase or hydrolase, and (iv) 3-hydroxypent-4-enoate decarboxylase, and wherein said microbial organism comprises at least one exogenous nucleic acid encoding an enzyme from said 1,3-butadiene pathway enzymes (i)-(iv) wherein said enzyme is expressed in a sufficient amount to produce 1,3-butadiene.
2. The non-naturally occurring microbial organism of claim 1 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding a 1,3-butadiene pathway enzyme.
3. The non-naturally occurring microbial organism of claim 1 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.
4. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
5. The non-naturally occurring microbial organism of claim 1 , wherein said non-naturally occurring microbial organism is a species of bacteria, yeast or fungus.
6. A method for producing 1,3-butadiene, comprising culturing a non-naturally occurring microbial organism according to claim 1 , under conditions and for a sufficient period of time to produce 1,3-butadiene.
7. The method of claim 6 , wherein said microbial organism comprises two, three, or four exogenous nucleic acids each encoding a 1,3-butadiene pathway enzyme.
8. The method of claim 6 , wherein said at least one exogenous nucleic acid is a heterologous nucleic acid.
9. The method of claim 6 , wherein said non-naturally occurring microbial organism is in a substantially anaerobic culture medium.
10. The method of claim 6 , wherein said non-naturally occurring microbial organism is a species of bacteria, yeast or fungus.