Chromatography media and method
View Patent ↗Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).
1. A non-woven bed of fibers having a cross-section comprising a body region defining a substantially longitudinal axis, and having a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling ion-exchange chromatography.
2. A housing comprising a packed bed of loose or fused cut fibers having a cross-section comprising a body region defining a substantially longitudinal axis, and having a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling ion-exchange chromatography.
3. A process for purifying
proteins, comprising;
providing a protein mixture,
contacting said protein mixture with a bed of fibers having a cross-section comprising a body region defining a substantially longitudinal axis, and having a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling a chromatography selected from the group consisting of ion-exchange functionality, hydrophobic interaction, and affinity chromatography,
washing said fiber media to remove unbound species,
compressing said fiber media, and
washing said compressed fiber media to extract bound protein.
4. Chromatography media comprising fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon anion-exchange functionality.
5. Chromatography media comprising fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon acrylic polymer functionality.
6. Chromatography media comprising fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling cation-exchange chromatography, wherein the surfaces of said fibers are modified with pendant sulfopropyl groups.
7. Chromatography media comprising fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon functionality enabling anion-exchange chromatography, wherein the surfaces of said fibers are modified with pendant trimethylammonium groups.
8. A housing comprising a packed bed of fibers having a cross-section comprising a region comprising a body region defining a substantially longitudinal axis, and a plurality of projections extending outwardly from said body region, said fibers having imparted thereon anion-exchange functionality, cation-exchange functionality, or acrylic polymer functionality.
9. The process of claim 3 , wherein said protein comprises a monoclonal antibody.