Method for the producing alpha-1, 6-branched alpha-1, 4-glucans from sucrose
View Patent ↗Nucleic acid molecules which encode a branching enzyme from a bacterium of the genus Neisseria , vectors, host cell, plant cells and plants containing said nucleic acid molecules as well as starch obtainable from the plants described are described. Furthermore, an in-vitro method for producing α-1,6-branched α-1,4-glucans on the basis of sucrose and a combination of enzymes of an amylosucrase and a branching enzyme as well as the α-1,6-branched α-1,4-glucans obtainable by said method are described.
1. Starch prepared by the process that comprises extracting starch from transgenic plants or parts thereof that comprise transgenic plant cell containing a nucleic acid molecule linked to regulatory sequences guaranteeing the transcription in plant cells, wherein said nucleic acid molecule encodes a branching enzyme from a bacterium of the genus Neisseria selected from the group consisting of
(a) nucleic acid molecules encoding a protein which comprises the amino acid sequence depicted in SEQ ID NO. 2;
(b) nucleic acid molecules comprising the coding region depicted in SEQ ID NO. 1;
(c) nucleic acid molecules encoding a protein which comprises the amino acid sequence encoded by the insert in plasmid DSM 12425;
(d) nucleic acid molecules comprising the coding region for a branching enzyme, which is contained in the insert of the plasmid DSM 12425; and
(e) nucleic acid molecules encoding a protein the sequence of which has, in the first 100 amino acids, a homology of at least 65% to the amino acid sequence depicted in SEQ ID NO. 2;
wherein said starch has a phosphate content that is at last 20% reduced as compared to starch from a corresponding wild type plant.
2. The starch according to claim 1 , wherein the composition of the starch is modified in such a way that it has at least one of the properties selected from the group consisting of an increased gel texture, a reduced phosphate content, a reduced peak viscosity, a reduced pastification temperature, a reduced size of starch granules and a modified distribution of side-chains when compared to the starch from corresponding wild type plant.
3. The starch according to claim 1 , wherein said starch has a modified distribution of side-chains in that the proportion of side-chains with a DP of 6 to 8 is increased by at least 25% in comparison to the proportion of side-chains with the corresponding degree of polymerization of amylopectin from a corresponding wild-type plant.
4. The starch according to claim 1 , wherein said protein sequence has a homology of at least 95% to the amino acid sequence depicted in SEQ ID NO. 2.