Stationary bubble reactors

1. A flexible horizontally disposed disposable bioreactor bag capable of holding nutrient media comprising:

a flexible top sheet, a porous flexible middle sheet, and a flexible bottom sheet, the porous flexible middle sheet tufted to the flexible bottom sheet to form a sparging compartment covering essentially an entire bottom surface of the bioreactor bag, wherein the flexible top sheet and the flexible bottom sheet are sealed together at their edges and the sparging compartment serves to aerate and mix nutrient media located in a nutrient media compartment formed between the flexible top sheet and the porous flexible middle sheet;

a gas inlet that extends through the nutrient media compartment to the sparging compartment to an inlet point near a central location of the flexible middle sheet to provide compressed gas to the sparging compartment;

at least one nutrient media inlet port in fluid communication with the nutrient media compartment; and

at least one compressed gas outlet port in fluid communication with the nutrient media compartment,

wherein the porous flexible middle sheet allows the compressed gas from the gas inlet to aerate the nutrient media compartment from the sparging compartment, and

wherein a plurality of pores of the flexible middle sheet are arranged so that pores located proximal to the inlet point near the central location of the flexible middle sheet are less densely dispersed than pores located proximal to an outer edge of the flexible middle sheet.

2. A disposable bioreactor bag comprising:

a) a flexible top sheet;

b) a flexible bottom sheet sealed together with the flexible top sheet at their edges;

c) a porous flexible middle sheet tufted to the flexible bottom sheet to form (i) a sparging compartment between the porous flexible middle sheet and the flexible bottom sheet, the sparging compartment covering essentially an entire bottom surface of the bag, and (ii) a nutrient media compartment between the porous flexible middle sheet and the flexible top sheet;

d) a gas inlet that extends through the nutrient media compartment to the sparging compartment to an inlet point near a central location of the flexible middle sheet to provide compressed gas to the sparging compartment;

e) at least one nutrient media inlet port in fluid communication with the nutrient media compartment; and

f) at least one compressed gas outlet port in fluid communication with the nutrient media compartment,

wherein the porous middle sheet allows the compressed gas from the gas inlet to aerate the nutrient media compartment from the sparging compartment, and

wherein a plurality of pores of the flexible middle sheet are arranged so that pores located proximal to the inlet point near the central location of the flexible middle sheet are less densely dispersed than pores located proximal to an outer edge of the flexible middle sheet.

3. The bioreactor of claim 1 or claim 2 , wherein said plurality of pores each has a diameter less than 100 μm in size.

4. The bioreactor of claim 1 or claim 2 , wherein said plurality of pores each has a diameter less than 10 pm in size.

5. The bioreactor of claim 1 , wherein the flexible bottom sheet includes a liquid drain port directly connected to the sparging compartment.

6. The bioreactor of claim 1 , wherein the gas inlet extends through the flexible top sheet to a center of the porous flexible middle sheet to provide compressed gas to the sparging compartment.

7. The bioreactor of claim 1 , wherein the porous flexible middle sheet is tufted to the flexible bottom sheet at a plurality of locations.

8. The bioreactor of claim 1 or claim 2 , wherein the middle sheet produces horizontal and vertical mixing.

9. The bioreactor of claim 2 , wherein the flexible bottom sheet includes a liquid drain port directly connected to the sparging compartment.

10. The bioreactor of claim 2 , wherein the gas inlet extends through the flexible top sheet to a center of the porous flexible middle sheet to provide compressed gas to the sparging compartment.

11. The bioreactor of claim 2 , wherein the porous flexible middle sheet is tufted to the flexible bottom sheet at a plurality of locations.