Compositions for bacterial mediated gene silencing and methods of using same
View Patent ↗Methods are described for the delivery of one or more small interfering RNAs (siRNAs) to a eukaryotic cell using a bacterium. Methods are also described for using this bacterium to regulate gene expression in eukaryotic cells using RNA interference, and methods for treating cancer of cell proliferative disorders. The bacterium includes one or more siRNAs or one or more DNA molecules encoding one or more siRNAs. Vectors are also described for use with the bacteria of the invention for causing RNA interference in eukaryotic cells.
1. An invasive bacterium comprising a prokaryotic vector comprising one or more DNA molecules encoding one or more siRNAs, at least one prokaryotic promoter, at least one Hly A gene and at least one Inv gene.
2. A prokaryotic vector comprising at least one DNA molecule encoding one or more siRNAs, at least one prokaryotic promoter, at least one H1yA gene and at least one Inv gene.
3. The invasive bacterium of claim 1 , wherein the invasive bacterium is non-pathogenic or non-virulent.
4. The invasive bacterium of claim 1 , wherein the invasive bacterium is an attenuated strain selected from Listeria, Shigella, Salmonella, E. Coli , and Bifidobacteriae.
5. The invasive bacterium of claim 4 , wherein the Salmonella is an attenuated strain of Salmonella typhimurium species.
6. The invasive bacterium of claim 5 , wherein the attenuated strain of Salmonella typhimurium is SL7207 or VNP20009.
7. The invasive bacterium of claim 4 , wherein the E. Coli is BM2710.