Device, system, and method including micro-patterned cell treatment array
View Patent ↗Devices, systems, or methods are disclosed herein for treatment of disease in a vertebrate subject. The device can include a quasi-planar substrate; and one or more laterally-mobile effector molecule types at least partially embedded within the quasi-planar substrate, wherein the one or more laterally-mobile effector molecule types is configured to interact with one or more cell types. The device can further include one or more sensors configured to detect at least one aspect of an interaction between the at least one of the one or more laterally-mobile effector molecule types and the one or more cell types; and a controller in communication with the one or more sensors, wherein the controller is configured to responsively initiate modification of at least one of the one or more laterally-mobile effector molecule types, the quasi-planar substrate, and the one or more cell types.
1. A method comprising
embedding two or more laterally-mobile effector molecule types at least partially within a quasi-planar substrate of a device,
transferring one or more cell types to the quasi-planar substrate of the device,
interacting at least one of the two or more laterally-mobile effector molecule types with the one or more cell types,
sensing at least one aspect of an interaction between the two or more laterally-mobile effector molecule types and the one or more cell types,
controlling, in response to the sensing, initiation of modification of at least one of the two or more laterally-mobile effector molecule types and the quasi-planar substrate, and
controlling, in response to the sensing, interaction of the at least one of the two or more laterally-mobile effector molecule types with the one or more cell types to activate, mature, differentiate, induce anergy in, load antigen to, electroporate, or transduce the one or more cell types.
2. The method of claim 1 , wherein at least one of the one or more effector molecule type is a ligand molecule.
3. The method of claim 1 , wherein at least one of the one or more effector molecule type is a receptor molecule.
4. The method of claim 1 , wherein at least one of the two or more effector molecule type is a cytokine.
5. The method of claim 1 , wherein the two or more laterally-mobile effector molecule types modified in response to the controlling is different from the two or more laterally-mobile effector molecule types sensed interacting with the one or more cell types.
6. The method of claim 1 , comprising interacting the one or more cell types with at least two of the two or more laterally-mobile effector molecule types such that the one or more cell types interact sequentially with the at least two of the two or more laterally-mobile effector molecule types.
7. The method of claim 1 , comprising interacting the one or more cell types with at least two of the one or more laterally-mobile effector molecule types such that the one or more cell types interact simultaneously with the at least two of the one or more laterally-mobile effector molecule types.
8. The method of claim 1 , wherein controlling movement further includes adding at least one of the two or more laterally-mobile effector molecule types to the quasi-planar substrate.
9. The method of claim 1 , wherein the quasi-planar substrate includes one or more discreet regions configured to include at least one of the two or more laterally-mobile effector molecule types.
10. The method of claim 1 , wherein controlling movement further includes removing at least one of the two or more laterally-mobile effector molecule types from the quasi-planar substrate.
11. The method of claim 1 , wherein controlling movement further includes moving at least one of the one or more laterally-mobile effector molecule types and the one or more cell types on the quasi-planar substrate.
12. The method of claim 1 , wherein controlling movement further includes removing at least one of the two or more laterally-mobile effector molecule types and the one or more cell types from the quasi-planar substrate.
13. The method of claim 1 , wherein the quasi-planar substrate includes a liquid configured as an interface with a cell type-carrying fluid.
14. The method of claim 13 , further comprising embedding at least one of the two or more laterally-mobile effector molecule types in the liquid of the quasi-planar substrate.
15. The method of claim 13 , wherein the quasi-planar substrate includes a membrane.
16. The method of claim 15 , wherein the membrane is bordered by a solid substrate.
17. The method of claim 15 , further comprising contacting a liquid surface or a solid surface of the quasi-planar substrate with the membrane.
18. The method of claim 1 , further comprising mobilizing at least one of the two or more laterally-mobile effector molecule types in essentially two dimensions on the quasi-planar substrate.
19. The method of claim 1 , further comprising bi-directionally mobilizing at least one of the one or more laterally-mobile effector molecule types in essentially one dimension on the quasi-planar substrate.
20. The method of claim 1 , wherein the quasi-planar substrate is configured to include one or more spatially-discrete portions.
21. The method of claim 20 , wherein each of the one or more spatially-discrete portions includes two or more identical of the two or more laterally-mobile effector molecule type.
22. The method of claim 20 , wherein each of the one or more spatially-discrete portions includes two or more different of the two or more laterally-mobile effector molecule type.
23. The method of claim 20 , wherein the one or more spatially-discrete portions include one or more of array sites or channels.
24. The method of claim 20 , further comprising fluidically connecting the one or more spatially-discrete portions.
25. The method of claim 24 , further comprising controlling communication between the one or more spatially discrete portions with one or more of static connections or dynamic connections.
26. The method of claim 25 , wherein the one or more of static connections or the dynamic connections are fluid connections.
27. The method of claim 1 , further comprising inducing a conformational change in at least one of the two or more laterally-mobile effector molecule types in response to interaction with the one or more cell types.
28. The method of claim 1 , wherein at least one of the two or more laterally-mobile effector molecule types is configured to bind and interact with a single cell type of the one or more cell types.
29. The method of claim 28 , further comprising identifying the single cell type by one or more sensors, and modifying the single cell type in response to action of the controller.
30. The method of claim 1 , further comprising spatially detecting at least one of the two or more laterally-mobile effector molecule types and the one or more cell types in one or more spatially-discrete portions of the quasi-planar substrate with one or more sensors.
31. The method of claim 30 , further comprising detecting one or more responsive interactions of at least one of the two or more laterally-mobile effector molecule types with the one or more cell types by the one or more sensors, wherein the one or more responsive interactions occur at the one or more spatially-discrete portions of the quasi-planar substrate.
32. The method of claim 30 , further comprising detecting a position of the one or more cell types bound to the quasi-planar substrate by the one or more sensors.
33. The method of claim 1 , further comprising introducing at least one of the one or more laterally-mobile effector molecule types to the quasi-planar substrate by response of the controller.
34. The method of claim 1 , further comprising removing at least one of the two or more laterally-mobile effector molecule types from the quasi-planar substrate by response of the controller.
35. The method of claim 1 , wherein at least one of the two or more laterally-mobile effector molecule types is configured to increase binding of the one or more cell types to the quasi-planar substrate.
36. The method of claim 1 , wherein at least one of the one or more laterally-mobile effector molecule types is configured to decrease binding of the one or more cell types to the quasi-planar substrate.
37. The method of claim 1 , wherein the controlling is configured to direct motion of at least one of the two or more laterally-mobile effector molecule types toward or away from the one or more cell types.
38. The method of claim 1 , wherein the controlling is configured to direct application of fluidic force or motional force to at least one of the two or more laterally-mobile effector molecule types or the quasi-planar substrate.
39. The method of claim 1 , wherein the controlling is configured to direct alteration of connectivity of the quasi-planar substrate.
40. The method of claim 39 , wherein the controlling is configured to direct alteration of MEMS barriers on the quasi-planar substrate.
41. The method of claim 1 , wherein the controlling is configured to direct alteration of movement of the one or more cell types between one or more spatially discrete portions of the quasi-planar substrate.
42. The method of claim 1 , wherein the controlling is configured to direct alteration of rotation of the one or more cell types to a new orientation.
43. The method of claim 1 , wherein the controlling is configured to respond to binding of the one or more cell types at one region of the quasi-planar substrate by directing alteration of a configuration of at least one of the two or more laterally-mobile effector molecule types at a second region on the quasi-planar substrate.
44. The method of claim 1 , further comprising receiving and processing one or more cell types in one or more fluids from a vertebrate subject through one or more fluid bypasses of the device and returning processed fluids through the one or more fluid bypasses into the vertebrate subject through the one or more fluid bypasses.