Oxygenated demineralized bone matrix for use in bone growth
An improved composition for inducing bone growth is provided that is a combination of at least DBM and an oxygen carrier. Injection/implantation of a composition of DBM and an oxygen carrier (e.g. a perfluorocarbon) results in enhancement of bone formation compared to DBM alone.
1 . A composition, comprising:
an oxygen carrier; and
demineralized bone matrix (DBM).
2 . The composition of claim 1 , wherein the oxygen carrier comprises a perfluorocarbon.
3 . The composition of claim 2 , wherein the perfluorocarbon is selected from the group consisting of perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, octafluoropropane, perfluorohexane, perfluorodecalin, perfluorodichlorooctane, perfluorodecane, perfluorotripropylamine, perfluorotrimethylcyclohexane, perfluoroperhydrophenanthrene, perfluoromethyladamantane, perfluorodimethyladamantane, perfluoromethyldecaline, perfluorofluorene, diphenyldimethylsiloxane, hydrogen-rich monohydroperfluorooctane, alumina-treated perfluorooctane, and mixtures thereof.
4 . The composition of claim 2 , wherein the perfluorocarbon is PFTBA.
5 . The composition of claim 4 , wherein the PFTBA is in a range from about 5% to 20% (w/v).
6 . The composition of claim 5 , wherein the PFTBA is 10% (w/v).
7 . The composition of claim 1 , wherein DBM is in the form of putty, gel, strips, paste, sheets, circular grafts, fibers, and matrices.
8 . The composition of claim 7 , wherein the DBM is in the form of putty.
9 . The composition of claim 1 , further comprising at least one selected from the group consisting of autologous bone chips, allograft bone chips, growth factors, fibrin, collagen, synthetic scaffolds, and bone marrow-derived stem cells.
10 . The composition of claim 1 for enhancing bone growth.
11 . A method of inducing bone growth comprising:
mixing an oxygen carrier and DBM to form a mixture; and
implanting an effective amount of the mixture into a subject at a target site.
12 . The method of claim 11 , wherein the oxygen carrier is a perfluorocarbon.
13 . The method of claim 12 , wherein the perfluorocarbon is selected from the group consisting of perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, perfluorobutylamine (PFTBA), perfluorooctylbromide (PFOB), perfluoro-n-octane, octafluoropropane, perfluorohexane, perfluorodecalin, perfluorodichlorooctane, perfluorodecane, perfluorotripropylamine, perfluorotrimethylcyclohexane, perfluoroperhydrophenanthrene, perfluoromethyladamantane, perfluorodimethyladamantane, perfluoromethyldecaline, perfluorofluorene, diphenyldimethylsiloxane, hydrogen-rich monohydroperfluorooctane, alumina-treated perfluorooctane, and mixtures thereof.
14 . The method of claim 12 , wherein the perfluorocarbon is PFTBA.
15 . The method of claim 14 , wherein the PFTBA is 10% (w/v).
16 . The method of claim 11 , wherein DBM is in the form of putty, gel, strips, paste, sheets, circular grafts, fibers, and matrices.
17 . The method of claim 16 , wherein the DBM is in the form of putty.
18 . The method of claim 11 , wherein the mixture further comprises at least one selected from the group consisting of autologous bone chips, allograft bone chips, growth factors, fibrin, collagen, synthetic scaffolds, and bone marrow-derived stem cells.