Selective 3D biopatterning
View Patent ↗Methods, systems, and articles for selective three dimensional (3D) biopatterning are disclosed. A biological target may be imaged and a selected area of the image may define a desired pattern for guiding the emission of EM radiation into the biological target. Two or more groups of photosensitive elements responsive to different activation wavelengths may be provided. The photosensitive elements may be selectively activated on or within the biological target based on location and activation wavelength in order to guide cell differentiation, adhesion of growth factors to a scaffold, release of growth factors, and/or deletion of cells.
1. A method for biopatterning a biological target, the method including:
obtaining a radiation pattern to be applied to an area of the biological target, wherein the biological target includes a first group of photosensitive elements, and wherein the first group of photosensitive elements is responsive to a first activation wavelength;
radiating a first light beam of a first shape into the area of the biological target, wherein the first light beam includes a first radiation dose including the first activation wavelength, and wherein the first radiation dose does not satisfy an activation threshold for activating the first group of photosensitive elements;
radiating a second light beam of a second shape into the area of the biological target, wherein the second light beam includes a second radiation dose including the first activation wavelength, wherein the second radiation dose does not satisfy the activation threshold, and wherein a combination of the first dose and the second dose satisfies the activation threshold; and
activating the first group of photosensitive elements located within an intersection of the first light beam and the second light beam in the area of the biological target responsive to satisfaction of the activation threshold, wherein the intersection of the first light beam and the second light beam has a contour that matches at least a portion of the radiation pattern.
2. The method of claim 1 , further including:
selecting, from an image of the biological target, one or more contours of the area in which the radiation pattern is to be applied.
3. The method of claim 2 , further including:
scanning the biological target to generate scan data; and
generating the image of the biological target based on the scan data.
4. The method of claim 2 , wherein said obtaining the radiation pattern comprises selecting the one or more contours of the area, and wherein the radiation pattern is defined by the one or more contours.
5. The method of claim 1 , wherein the radiation pattern comprises a three-dimensional (3D) pattern, and wherein the contour of the intersection matches at least a portion of the 3D pattern.
6. The method of claim 1 , wherein the first activation wavelength is in the range of 1 mm to 760 nm, 760 nm to 380 nm, or 380 nm to 10 nm.
7. The method of claim 1 , wherein the first group of photosensitive elements located within the intersection is activated to adhere to the biological target.
8. The method of claim 1 , wherein the first group of photosensitive elements located within the intersection is activated to adhere to the biological target to form a 3D structure in the area of the biological target.
9. The method of claim 1 , wherein the first group of photosensitive elements comprises photo-adhesive molecules, photo-adhesive biochemical factors, or nanoparticles.
10. The method of claim 1 , wherein the biological target comprises a tissue, an organ, or a scaffold.
11. The method of claim 1 , wherein at least some of the first group of photosensitive elements are each coupled to one or more biochemical factors, and wherein the photosensitive elements are configured to release the one or more biochemical factors in response to radiation of the first activation wavelength.
12. The method of claim 11 , wherein the one or more biochemical factors comprises a growth factor, a nucleic acid molecule, a ribonucleic acid molecule, or a protein.
13. The method of claim 1 , wherein the first group of photosensitive elements includes nanoparticles, and wherein a targeting molecule is coupled to an outer surface of a particular nanoparticle.
14. The method of claim 1 , wherein the biological target further includes a second group of photosensitive elements, and wherein the second group of photosensitive elements is responsive to a second activation wavelength, the method further including:
radiating a third light beam of a third shape into the area of the biological target, wherein the third light beam includes a third radiation dose including the second activation wavelength, and wherein the third radiation dose does not satisfy a second activation threshold for activating the second group of photosensitive elements;
radiating a fourth light beam of a fourth shape into the area of the biological target, wherein the fourth light beam includes a fourth radiation dose including the second activation wavelength, wherein the fourth radiation dose does not satisfy the second activation threshold, and wherein a combination of the third dose and the fourth dose satisfies the second activation threshold; and
activating the second group of photosensitive elements located within a second intersection of the third light beam and the fourth light beam in the area of the biological target, wherein the second intersection has a contour that matches at least another portion of the radiation pattern.
15. The method of claim 1 , wherein the obtaining is performed by a computing source, wherein the radiating the first light beam is performed by a first radiation source, wherein the radiating the second light beam is performed by a second radiation source.