Methods and articles for detecting deoxyribonuclease activity
The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms.
1. A method of detecting a microorganism, comprising:
providing a dry composition and a sample suspected of containing a microorganism;
wherein the dry composition comprises a cold water-soluble gelling agent and a DNase indicator system including methyl green and DNA;
contacting a predetermined volume of aqueous liquid comprising the sample with the dry composition to form a hydrogel;
incubating the hydrogel for a period of time; and
detecting a microorganism;
wherein, after contact with the predetermined volume of aqueous liquid, the concentration of methyl green in the hydrogel is about 5 μg/ml to 20 μg/mL.
2. The method of claim 1 , wherein the dry composition further comprises a nutrient, a selective agent, or an indicator.
3. The method of claim 2 , wherein the selective agent comprises an antibiotic.
4. The method of claim 1 , further comprising the step of providing a nutrient, a selective agent, an indicator, or any combination of two or more of the foregoing; wherein the aqueous liquid comprising the sample further comprises the nutrient, the selective agent, the indicator, or any combination of two or more of the foregoing.
5. The method of claim 1 , further comprising:
providing an imaging system; and
obtaining an image of the hydrogel with the imaging system;
wherein detecting a microorganism comprises displaying, printing, or analyzing the image of the hydrogel.
6. The method of claim 1 , further comprising enumerating one or more types of microorganisms.