Immobilized biologically active entities having a high degree of biological activity
View Patent ↗The present invention relates to immobilized biologically active entities having heparin cofactor II binding activity.
1. A medical device comprising:
a substrate material;
a polymeric covering material attached to at least a portion of a surface of said substrate material, a plurality of biologically active end-point attached entities having heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ) covalently attached to at least a portion of said polymeric covering material; and
a biologically compatible composition combined with said polymeric covering material,
wherein said biologically compatible composition has antithrombin Ill binding activity.
2. The medical device of claim 1 wherein said biologically compatible composition is covalently combined with said polymeric covering material.
3. The medical device of claim 1 wherein said biologically compatible composition is non-covalently combined with said polymeric covering material.
4. The medical device of claim 1 wherein said plurality of biologically active end-point attached entities comprises a glycosaminoglycan.
5. The medical device of claim 1 wherein said plurality of biologically active entities comprises end-point attached dermatan disulfate.
6. The medical device of claim 1 wherein at least a portion of said biologically compatible composition is released from said medical device in a 0.15M phosphate buffer solution having a temperature of about thirty-seven degrees centigrade and a substantially neutral pH.
7. The medical device of claim 1 wherein said biologically compatible composition comprises an organic compound.
8. The medical device of claim 7 wherein said organic compound is a polysaccharide.
9. The medical device of claim 8 wherein said polysaccharide is a glycosaminoglycan.
10. The medical device of claim 7 wherein said polysaccharide is dextran.
11. The medical device of claim 8 wherein said polysaccharide is dextran sulfate.
12. The medical device of claim 1 wherein said biologically compatible composition is polyethylene glycol.
13. The medical device of claim 1 wherein said biologically compatible composition is an antiproliferative agent.
14. The medical device of claim 13 wherein said antiproliferative agent is dexamethasone.
15. The medical device of claim 1 wherein said biologically compatible composition comprises a synthetic non-polar molecule.
16. The medical device of claim 1 wherein said biologically compatible composition comprises an inorganic compound.
17. The medical device of claim 16 wherein said inorganic compound comprises a phosphate.
18. The medical device of claim 1 wherein said plurality of biologically active end-point attached entities have a heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ) substrate material following sterilization of said substrate material.
19. The medical device of claim 1 wherein said plurality of biologically active end-point attached entities have a heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ) substrate material following compaction and expansion of said substrate material.