Anti-hemagglutinin antibody compositions and methods of use thereof
The present invention provides novel human anti-Influenza antibodies and related compositions and methods. These antibodies are used in the prevention, inhibition, neutralization, diagnosis, and treatment of influenza infection.
1. A composition comprising:
a) an isolated human monoclonal antibody, wherein said antibody comprises a VH CDR1 region comprising the amino acid sequence of DYSWN (SEQ ID NO: 34), a VH CDR2 region comprising the amino acid sequence of DTLHNGYTNYNPSLRG (SEQ ID NO: 35), a VH CDR3 region comprising the amino acid sequence of GSGGYGGFDYFGKLRTWDF (SEQ ID NO: 36), a VL CDR1 region comprising the amino acid sequence of RASQGIRNDLG (SEQ ID NO: 41), a VL CDR2 region comprising the amino acid sequence of GASSLQS (SEQ ID NO: 42), and a VL CDR3 region comprising the amino acid sequence of LQHNSYPYT (SEQ ID NO: 43), and
b) an isolated human monoclonal antibody, wherein said antibody comprises a VH CDR1 region comprising the amino acid sequence of NYGIS (SEQ ID NO: 174), a VH CDR2 region comprising the amino acid sequence of GIIPLFGAAKYAQKFQG (SEQ ID NO: 175), a VH CDR3 region comprising the amino acid sequence of APRVYEYYFDQ (SEQ ID NO: 176), a VL CDR1 region comprising the amino acid sequence of RASQSVSSSQLA (SEQ ID NO: 157), a VL CDR2 region comprising the amino acid sequence of GASTRAT (SEQ ID NO: 181), and a VL CDR3 region comprising the amino acid sequence of QQYSTSPPT (SEQ ID NO: 182), wherein at least one of the antibodies is directly conjugated to either a detectable label or therapeutic agent.
2. A composition comprising:
a) an isolated human monoclonal antibody comprising a heavy chain variable sequence comprising the amino acid sequence of SEQ ID NO: 33 and a light chain variable sequence comprising the amino acid sequence of SEQ ID NO: 40; and
b) an isolated human monoclonal antibody comprising a heavy chain variable sequence comprising the amino acid sequence of SEQ ID NO: 173 and a light chain variable sequence comprising the amino acid sequence of SEQ ID NO: 180,
wherein at least one of the antibodies is directly conjugated to either a detectable label or therapeutic agent.
3. A pharmaceutical composition comprising the composition of any one of claims 1 or 2 and a pharmaceutical carrier.
4. The pharmaceutical composition of claim 3 , further comprising an anti-viral drug, a viral entry inhibitor or a viral attachment inhibitor.
5. The pharmaceutical composition of claim 4 , wherein said anti-viral drug is a neuraminidase inhibitor, a HA inhibitor, a sialic acid inhibitor or an M2 ion channel inhibitor.
6. The pharmaceutical composition of claim 5 , wherein said neuraminidase inhibitor is zanamivir or oseltamivir phosphate.
7. A passive immunization composition comprising the composition of any one of claims 1 or 2 .