Designer osteogenic proteins
The invention relates to novel designer osteogenic proteins having altered affinity for a cognate receptor, nucleic acids encoding the same, and methods of use therefor. More preferably, the novel designer osteogenic proteins are designer BMPs and have altered affinity for a cognate BMP receptor. The designer BMPs demonstrate altered biological characteristics and provide potential useful novel therapeutics.
1. A designer osteogenic protein comprising the amino acid sequence of any one of SEQ ID NOs: 36, 37, and 50.
2. A method of producing the protein of claim 1 , the method comprising introducing a nucleic acid encoding the protein into a host cell, culturing the cell under conditions where the protein is produced, and purifying the protein.
3. An isolated nucleic acid molecule comprising a nucleotide sequence encoding the designer osteogenic protein of claim 1 .
4. A method of treating a bone disease associated with bone loss in a patient in need thereof, the method comprising administering an effective amount of a designer osteogenic protein of claim 1 to said patient, thereby treating bone disease in said patient.
5. A method of inducing bone formation in a tissue, said method comprising contacting said tissue with a designer osteogenic protein of claim 1 , thereby inducing bone formation in said tissue.
6. A designer osteogenic protein comprising the amino acid sequence of SEQ ID NO: 36.
7. A designer osteogenic protein comprising the amino acid sequence of SEQ ID NO: 50.
8. A designer osteogenic protein comprising the amino acid sequence of SEQ ID NO: 37.
9. A designer osteogenic protein comprising the amino acid sequence of SEQ ID NO: 71.
10. A designer osteogenic protein comprising the amino acid sequence of SEQ ID NO: 73.