Method for screening of antiviral agents

1. A method for screening for an antiviral agent capable of blocking a viral viroporin comprising

inducing expression of a viral viroporin fragment in a Single Protein Production system of Escherichia coli ( E. coli );

contacting the E. coli with a test compound; and

determining whether expression of the viral viroporin fragment is rescued,

wherein rescue of the expression of the viral viroporin fragment indicates that the test compound blocks the viral viroporin.

2. The method of claim 1 , wherein expression of the viral viroporin fragment is determined via a reporter protein.