Microfluidic free interface diffusion techniques
View Patent ↗A static fluid and a second fluid are placed into contact along a microfluidic free interface and allowed to mix by diffusion without convective flow across the interface. In accordance with one embodiment of the present invention, the fluids are static and initially positioned on either side of a closed valve structure in a microfluidic channel having a width that is tightly constrained in at least one dimension. The valve is then opened, and no-slip layers at the sides of the microfluidic channel suppress convective mixing between the two fluids along the resulting interface. Applications for microfluidic free interfaces in accordance with embodiments of the present invention include, but are not limited to, protein crystallization studies, protein solubility studies, determination of properties of fluidics systems, and a variety of biological assays such as diffusive immunoassays, substrate turnover assays, and competitive binding assays.
1. A system for analyzing a property of a target material, the system comprising:
a structure comprising:
a first microfabricated region configured to contain a volume of a first static fluid including a target;
a second microfabricated region configured to contain a volume of a second static fluid including an analyte known to bind to the target;
a valve actuable to place the volume of the first fluid in diffusive communication with the volume of the second fluid across a free microfluidic interface; and
a detector configured to detect a presence of the analyte in the first microfabricated region.
2. The system of claim 1 wherein:
the first microfabricated region comprises a first chamber in fluid communication with a first channel portion restricted in at least one dimension relative to dimensions of the first chamber;
the second microfabricated region comprises a second chamber in fluid communication with a second channel portion restricted in at least one dimension relative to dimensions of the second chamber;
the valve comprises an elastomer membrane deflectable into the channel between the first channel portion and the second channel portion; and
the detector is configured to detect a rate of increase of the concentration of the analyte in the first chamber over time.
3. The system of claim 1 wherein:
the first microfabricated region comprises a first microfabricated channel portion restricted in at least one dimension;
the second microfabricated region comprises a second microfabricated channel portion restricted in the at least one dimension;
the valve comprises an elastomer membrane deflectable into the microfabricated channel between the first channel portion and the second channel portion; and
the detector is configured to detect a concentration of the analyte over a distance from the valve in the first chamber.
4. The system of claim 1 wherein the detector is configured to detect at least one of fluorescence, refractive index, colorimetry, surface plasmon resonance, and conductivity.