Passive methods for anti-microbial biological meshes
View Patent ↗Tissue matrices having anti-microbial properties are provided. In certain embodiments, the tissue matrices include cationic anti-microbial agents that form a stable bond with the tissue matrices without adversely affecting the biologic properties of the tissue matrices.
1. An anti-microbial tissue matrix prepared by:
selecting a collagen-based tissue; and
contacting the tissue with an aqueous solution containing a cationic anti-microbial agent, wherein the agent is stably bound to a tissue matrix within the collagen-based tissue by passive adsorption in the aqueous solution, and wherein the tissue has been exposed to E-beam radiation.
2. The anti-microbial tissue matrix of claim 1 , wherein the aqueous solution is non-denaturing.
3. The anti-microbial tissue matrix of claim 1 , wherein the collagen-based tissue is an acellular tissue.
4. The anti-microbial tissue matrix of claim 1 , wherein the collagen-based tissue comprises an acellular dermal tissue.
5. The anti-microbial tissue matrix of claim 1 , wherein the collagen-based tissue comprises an acellular porcine dermal tissue.
6. The anti-microbial tissue matrix of claim 1 , wherein the anti-microbial compound comprises benzethonium chloride, chlorhexidine, benzalkonium chloride, silver nitrate, poly-hexa-methylene biguanide hydrochloride, cationic peptides, chitosan, or quaternary ammonium compounds.
7. The anti-microbial tissue matrix of claim 1 , wherein the collagen-based tissue is not cross-linked or denatured during binding of the anti-microbial agent.
8. The anti-microbial tissue matrix of claim 1 , wherein the anti-microbial agent is not covalently modified by binding to the collagen-based tissue.
9. The anti-microbial tissue matrix of claim 1 , wherein the tissue matrix retains anti-microbial properties for at least two weeks after binding the antimicrobial agent.
10. The anti-microbial tissue matrix of claim 1 , prepared by contacting the collagen-based tissue with the aqueous solution containing the cationic anti-microbial agent for at least four hours at room temperature.
11. The anti-microbial tissue matrix of claim 1 , wherein the antimicrobial agent remains bound to the tissue matrix when the tissue matrix is placed in a non-denaturing aqueous solution.
12. The anti-microbial tissue matrix of claim 1 , wherein the antimicrobial agent remains bound to the tissue matrix when the tissue matrix is placed in PBS.
13. The anti-microbial tissue matrix of claim 1 , wherein the bound anti-microbial agent retains greater than 95% of its activity when the tissue matrix is placed in PBS for two weeks at room temperature.
14. The anti-microbial tissue matrix of claim 1 , wherein the bound anti-microbial agent retains greater than 75% of its activity when the tissue matrix is placed in PBS for two weeks at room temperature.
15. An anti-microbial tissue matrix comprising:
a collagen-based tissue; and
at least one cationic anti-microbial agent that is stably bound to the collagen-based tissue by passive adsorption in an aqueous solution, and wherein the collagen-based tissue has been exposed to E-beam radiation.
16. The anti-microbial tissue matrix of claim 15 , wherein binding of the anti-microbial agent to the collagen-based tissue occurs in a non-denaturing aqueous solution.
17. The anti-microbial tissue matrix of claim 15 , wherein the collagen-based tissue comprises an acellular dermal tissue.
18. The anti-microbial tissue matrix of claim 15 , wherein the collagen-based tissue comprises an acellular porcine dermal tissue.
19. The anti-microbial tissue matrix of claim 15 , wherein the anti-microbial compound comprises at least one of benzethonium chloride, chlorhexidine, benzalkonium chloride, silver nitrate, poly-hexa-methylene biguanide hydrochloride, cationic peptides, chitosan, or quaternary ammonium compounds.
20. The anti-microbial tissue matrix of claim 15 , wherein the collagen-based tissue is not cross-linked or denatured during binding of the anti-microbial agent.
21. The anti-microbial tissue matrix of claim 15 , wherein the anti-microbial agent is not covalently modified by binding to the collagen-based tissue.
22. The anti-microbial tissue matrix of claim 15 , wherein the tissue matrix retains anti-microbial properties for at least two weeks after binding the anti-microbial agent.
23. The anti-microbial tissue matrix of claim 15 , prepared by contacting the collagen-based tissue with the aqueous solution containing the cationic anti-microbial agent for at least four hours at room temperature.
24. The anti-microbial tissue matrix of claim 15 , wherein the antimicrobial agent remains bound to the tissue matrix when the tissue matrix is placed in a non-denaturing aqueous solution.
25. The anti-microbial tissue matrix of claim 15 , wherein the antimicrobial agent remains bound to the tissue matrix when the tissue matrix is placed in PBS.
26. The anti-microbial tissue matrix of claim 15 , wherein the bound anti-microbial agent retains greater than 95% of its activity when the tissue matrix is placed in PBS for two weeks at room temperature.
27. The anti-microbial tissue matrix of claim 15 , wherein the bound anti-microbial agent retains greater than 75% of its activity when the tissue matrix is placed in PBS for two weeks at room temperature.