Nucleic acid molecules encoding neutralising antibodies having specificity for human IL-17
View Patent ↗The invention relates to an antibody molecule having specificity for antigenic determinants of IL-17, therapeutic uses of the antibody molecule and methods for producing said antibody molecule.
1. An isolated DNA molecule encoding the heavy chain of a neutralising antibody having specificity for human IL-17, wherein the variable domain of said heavy chain comprises the sequence given in SEQ ID NO: 5 for CDR-H1, the sequence given in SEQ ID NO: 6 for CDR-H2, and the sequence given in SEQ ID NO: 7 for CDR-H3.
2. The isolated DNA molecule of claim 1 comprising the sequence given in SEQ ID NO: 12.
3. The isolated DNA molecule of claim 1 comprising the sequence given in SEQ ID NO: 15.
4. A cloning or expression vector comprising the DNA molecule according to claim 1 .
5. An isolated host cell comprising the cloning or expression vector according to claim 4 .
6. An isolated DNA molecule, encoding the heavy chain of a neutralizing antibody having specificity for human IL-17, wherein the variable domain of said heavy chain comprises the sequence gH11 (SEQ ID NO: 11).
7. An isolated DNA molecule encoding the light chain of a neutralising antibody having specificity for human IL-17, comprising the nucleic acid sequence given in SEQ ID NO:14.
8. The isolated DNA molecule of claim 7 comprising the sequence given in SEQ ID NO: 17.
9. A cloning or expression vector comprising the DNA molecule according to claim 7 .
10. An isolated host cell comprising the cloning or expression vector according to claim 9 .
11. An isolated DNA molecule, encoding the light chain of a neutralizing antibody having specificity for human IL-17, wherein the variable domain of said light chain comprises the sequence gL3 (SEQ ID NO: 13).
12. A cloning or expression vector comprising an isolated DNA molecule encoding an antibody heavy chain variable domain and a light chain variable domain, wherein
(a) the heavy chain variable domain comprises the CDRs of SEQ ID NO:5, SEQ ID NO:6 and SEQ ID NO:7; and
(b) the light chain variable domains comprises the CDRs of SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10.
13. The vector according to claim 12 wherein the isolated DNA molecule comprises the nucleic acid sequence of SEQ ID NO: 19.
14. An isolated host cell comprising the cloning or expression vector according to claim 13 .
15. A process for the production of a neutralising antibody having specificity for human IL-17 which is encoded by the vector of claim 13 comprising,
(a) culturing an isolated host cell comprising said vector; and
(b) isolating the antibody.
16. An isolated host cell comprising the cloning or expression vector according to claim 12 .
17. A process for the production of a neutralising antibody having specificity for human IL-17 and encoded by the vector of claim 12 comprising,
(a) culturing an isolated host cell comprising said vector; and
(b) isolating the antibody.