IP Library › Patent Application 13222509
Patent Application
App. No. 13/222,509

Modified Cell Lines for Increasing Lentiviral Titers

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Patent No.
US None
App. No.
13/222,509
Abstract

The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.

Claims (14)

1 . A method of enhancing lentiviral titers comprising modulating expression of a plurality of host genes in a packaging cell.

2 . The method of claim 1 wherein the genes are selected from the group consisting of Annexin2, Tsg101, DBR1, RNA Helicase A, SOCS1, and TRBP, Dicer, Drosha, DGCR8, Hrs, Tetherin, CDK13, TRIM-5alpha, PRMT6, miR-29a, miR-29b, and miR-29c.

3 . The method of claim 2 , wherein the cell is an HEK-293 cell.

4 . The method of claim 2 , wherein the cell is an HEK-293T cell.

5 . The method of claim 2 , wherein the plurality of host gene comprises and DGCR8 and either Hrs or Tetherin.

6 . The method of claim 2 , wherein the plurality of host genes comprises DGCR8 and Tsg101.

7 . The method of claim 2 , wherein the plurality of host genes comprises DGCR8 and Annexin2.

8 . The method of claim 2 , wherein the plurality of host genes comprises Hrs and Tetherin.

9 . The method of claim 2 , wherein the plurality of host genes comprises Hrs and Annexin 2.

10 . The method of claim 2 , wherein the plurality of host genes comprises Tetherin and Tsg101.

11 . The method of claim 2 , wherein the plurality of host genes comprises Tetherin and Annexin2.

12 . The method of claim 2 , wherein the plurality of host genes comprises Tsg and Annexin 2.

13 . The method of claim 2 , wherein the plurality of host genes comprises DGCR8, Hrs and Tetherin.

14 . The method of claim 2 , wherein the plurality of host genes comprises DGCR8, Hrs and Annexin 2.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 2, 2011
From: SPAYD, KATIE JANSEN; KARPILOW, JON; WAKEFIELD, JOHN K.
To: DHARMACON, INC.
Reel/Frame 026849/0131 →