IP Library › Granted Patent US 10,117,411

Granted Patent B2

US 10,117,411 · App. 13/244,049 · Granted Nov 6, 2018

Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use

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Quick Facts

Patent No.: US 10,117,411
App. No.: 13/244,049
Granted: Nov 6, 2018
Kind: B2

External Links

USPTO Patent Center↗ Full Patent PDF↗

Abstract

This disclosure concerns high-resolution mapping and candidate gene cloning of Rf4, a maize restorer of fertility gene that restores fertility to C-type cytoplasmic male sterility. The disclosure also relates to molecular markers that are tightly-linked to, or reside within, the Rf4 gene. In some embodiments, methods are provided whereby hybrid seeds may be produced from crosses of a male plant comprising nucleic acid molecular markers that are linked to or that reside within the Rf4 gene and a female plant carrying C-type CMS.

Claims (1333)

1. A maize plant comprising an Rf4 functional restorer gene for maize C-type cytoplasmic male sterility from restorer line XJH58,

wherein the plant comprises a marker nucleotide sequence selected from the group consisting of Marker ID Nos. 1-50:

B73 Chrom. 8

Position

Marker ID No.

Start

Stop

Marker

8885

11684

11883

12831

13168

13171

13179

13191

14191

15309

15639

15735

15744

15790

17395

18749

18796

18798

18799

19119

22230

22266

41082

41335

41944

44285

44387

45202

—

46136

46447

46912

46957

46959

46973

46975

46989

47001

47026

65062

65063

—

65438

GGT

66993

79739

82093

85155

85667

85782

85784

—

86079

86097

86113

86247

86653

—,

and

wherein the plant comprises a marker nucleotide sequence selected from the group consisting of SEQ ID NOs:6-9, SEQ ID NO:105, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NO:115, SEQ ID NOs:118-120, SEQ ID NO:123, SEQ ID NO:126, SEQ ID NO:134, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:138, SEQ ID NO:144, SEQ ID NO:149, SEQ ID NO:151, SEQ ID NO:160, SEQ ID NO:163, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:173, SEQ ID NO:177, SEQ ID NO:178, SEQ ID NO:183, SEQ ID NOs:189-191, SEQ ID NO:197, and the markers referred to as Marker ID Nos. 51-106:

B73 Chrom. 8

Position

Marker ID No.

Start

Stop

Marker

96248

97087

97177

GCC

97337

97338

97341

97371

97382

GGCGTACTTGCGCGCGGAC

(SEQ ID NO: 212)

97405

GTC

97411

97421

97433

—

97452

GTT

97673

97827

97962

97964

ATGCATTACTT (SEQ ID NO: 214)

97973

GTGCTATACTACCTAACCTA

(SEQ ID NO: 215)

97989

98188

98323

98326

—

98335

98350

98359

98367

98369

—

98393

98403

98426

98430

—

98468

TGGTTTCT

98489

98533

98540

98552

98557

98566

CCCCTGAACC (SEQ ID NO: 216)

98660

98679

98735

98811

98857

ATAT

98906

98927

98935

99026

99027

99037

—

99074

99079

99089

99091

99137

ATATT

99298

100

99418

101

99443

102

99447

103

99491

104

99622

CTAATGGT

105

99925

106

99962

2. The maize plant of claim 1 , wherein the plant comprises a marker nucleotide sequence selected from the group consisting of SEQ ID NO:8 and Marker ID Nos. 1-3, 10, 11, 20, 21, 25-27, 39, 40, 44-49, 51-53, 55, 56, 59, 61-64, 68, 74, 78, and 83.

3. The maize plant of claim 1 , wherein the plant comprises a marker nucleotide sequence selected from the group consisting of SEQ ID NO:105; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:115; SEQ ID NOs:118-120; SEQ ID NO:123; SEQ ID NO:126 and SEQ ID NO:134.

4. A hybrid maize plant that is an F 1 progeny plant having XJH58 and a second plant as parents, the progeny plant comprising a marker nucleotide sequence selected from the group consisting of SEQ ID NOs:6-9, SEQ ID NO:105, SEQ ID NO:109, SEQ ID NO:111, SEQ ID NO:115, SEQ ID NOs:118-120, SEQ ID NO:123, SEQ ID NO:126, SEQ ID NO:134, SEQ ID NO:135, SEQ ID NO:137, SEQ ID NO:138, SEQ ID NO:144, SEQ ID NO:149, SEQ ID NO:151, SEQ ID NO:160, SEQ ID NO:163, SEQ ID NO:164, SEQ ID NO:167, SEQ ID NO:173, SEQ ID NO:177, SEQ ID NO:178, SEQ ID NO:183, SEQ ID NOs:189-191, SEQ ID NO:197, and the markers referred to as Marker ID Nos. 1-106:

B73 Chrom. 8

Position

Marker ID No.

Start

Stop

Marker

8885

11684

11883

12831

13168

13171

13179

13191

14191

15309

15639

15735

15744

15790

17395

18749

18796

18798

18799

19119

22230

22266

41082

41335

41944

44285

44387

45202

—

46136

46447

46912

46957

46959

46973

46975

46989

47001

47026

65062

65063

—

65438

GGT

66993

79739

82093

85155

85667

85782

85784

—

86079

86097

86113

86247

86653

—

96248

97087

97177

GCC

97337

97338

97341

97371

97382

GGCGTACTTGCGCGCGGAC

(SEQ ID NO: 212)

97405

GTC

97411

97421

97433

—

97452

GTT

97673

97827

97962

97964

ATGCATTACTT (SEQ ID NO: 214)

97973

GTGCTATACTACCTAACCTA

(SEQ ID NO: 215)

97989

98188

98323

98326

—

98335

98350

98359

98367

98369

—

98393

98403

98426

98430

—

98468

TGGTTTCT

98489

98533

98540

98552

98557

98566

CCCCTGAACC (SEQ ID NO: 216)

98660

98679

98735

98811

98857

ATAT

98906

98927

98935

99026

99027

99037

—

99074

99079

99089

99091

99137

ATATT

99298

100

99418

101

99443

102

99447

103

99491

104

99622

CTAATGGT

105

99925

106

99962

5. The F 1 hybrid maize plant of claim 4 , wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO:8 and Marker ID Nos. 1-3, 10, 11, 20, 21, 25-27, 39, 40, 44-49, 51-53, 55, 56, 59, 61-64, 68, 74, 78, and 83.

6. The F 1 hybrid maize plant of claim 4 , wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO:105; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:115; SEQ ID NOs:118-120; SEQ ID NO:123; SEQ ID NO:126 and SEQ ID NO:134.

7. The F 1 hybrid maize plant of claim 4 , comprising some but not all of the nucleotide sequences selected from the group consisting of SEQ ID NOs:6-9; SEQ ID NO:105; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:115; SEQ ID NOs:118-120; SEQ ID NO:123; SEQ ID NO:126; SEQ ID NO:134; SEQ ID NO:135; SEQ ID NO:137; SEQ ID NO:138; SEQ ID NO:144; SEQ ID NO:149; SEQ ID NO:151; SEQ ID NO:160; SEQ ID NO:163; SEQ ID NO:164; SEQ ID NO:167; SEQ ID NO:173; SEQ ID NO:177; SEQ ID NO:178; SEQ ID NO:183; SEQ ID NOs:189-191; SEQ ID NO:197; and the markers referred to as polymorphism ID nos. 1-106 in Table 3.

8. The maize plant of claim 1 , wherein the maize plant is an inbred maize plant.

9. The inbred maize plant of claim 8 , wherein the plant comprises a marker nucleotide sequence selected from the group consisting of SEQ ID NO:8 and Marker ID Nos. 1-3, 10, 11, 20, 21, 25-27, 39, 40, 44-49, 51-53, 55, 56, 59, 61-64, 68, 74, 78, and 83.

10. The inbred maize plant of claim 8 , wherein the plant comprises a marker nucleotide sequence selected from the group consisting of SEQ ID NO:105; SEQ ID NO:109; SEQ ID NO:111; SEQ ID NO:115; SEQ ID NOs:118-120; SEQ ID NO:123; SEQ ID NO:126 and SEQ ID NO:134.

11. A population consisting of at least 126 maize plants of claim 1 , wherein the population does not comprise a semi-fertile maize plant.

12. A maize plant comprising an Rf4 functional restorer gene for maize C-type cytoplasmic male sterility from restorer line XJH58,

wherein the plant comprises a genetic marker selected from the group consisting of Marker ID Nos. 1-50:

B73 Chrom. 8

Position

Marker ID No.

Start

Stop

Marker

8885

11684

11883

12831

13168

13171

13179

13191

14191

15309

15639

15735

15744

15790

17395

18749

18796

18798

18799

19119

22230

22266

41082

41335

41944

44285

44387

45202

—

46136

46447

46912

46957

46959

46973

46975

46989

47001

47026

65062

65063

—

65438

GGT

66993

79739

82093

85155

85667

85782

85784

—

86079

86097

86113

86247

86653

—,

and

wherein the plant comprises a genetic marker selected from the group consisting of the markers referred to as Marker ID Nos. 51-106:

B73 Chrom. 8

Position

Marker ID No.

Start

Stop

Marker

96248

97087

97177

GCC

97337

97338

97341

97371

97382

GGCGTACTTGCGCGCGGAC

(SEQ ID NO: 212)

97405

GTC

97411

97421

97433

—

97452

GTT

97673

97827

97962

97964

ATGCATTACTT (SEQ ID NO: 214)

97973

GTGCTATACTACCTAACCTA

(SEQ ID NO: 215)

97989

98188

98323

98326

—

98335

98350

98359

98367

98369

—

98393

98403

98426

98430

—

98468

TGGTTTCT

98489

98533

98540

98552

98557

98566

CCCCTGAACC (SEQ ID NO: 216)

98660

98679

98735

98811

98857

ATAT

98906

98927

98935

99026

99027

99037

—

99074

99079

99089

99091

99137

ATATT

99298

100

99418

101

99443

102

99447

103

99491

104

99622

CTAATGGT

105

99925

106

99962

13. The maize plant of claim 12 , wherein the plant comprises a genetic marker selected from the group consisting of Marker ID Nos. 73-106.

14. The maize plant of claim 13 , wherein the maize plant is an inbred maize plant.

15. The maize plant of claim 13 , wherein the maize plant is a hybrid maize plant that is a progeny plant having XJH58 and a second plant as parents.

16. A population consisting of at least 126 maize plants of claim 13 , wherein the population does not comprise a semi-fertile maize plant.

17. The maize plant of claim 12 , wherein the plant comprises in its genome a polynucleotide at least 95% identical to SEQ ID NO:217.

18. The maize plant of claim 17 , wherein the plant comprises in its genome a polynucleotide at least 99% identical to SEQ ID NO:217.

19. The maize plant of claim 18 , wherein the plant comprises in its genome the polynucleotide of SEQ ID NO:217.

Assignments (2)

CHANGE OF NAME Recorded Nov 8, 2021

From: DOW AGROSCIENCES LLC

To: CORTEVA AGRISCIENCE LLC

Reel/Frame 058044/0184 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 17, 2018

From: REN, RUIHUA; KUMPATLA, SIVA P; ZHENG, PEIZHONG; NAGEL, BRUCE A.; CUTTER, GARY; GREENE, THOMAS W.; THOMPSON, STEVEN A.

To: DOW AGROSCIENCES LLC

Reel/Frame 046888/0465 →

Cited By (1)

US 12,392,000