Method of preparing porous carbonate apatite from natural bone
View Patent ↗A method for preparing carbonate apatite from natural bones. The method includes obtaining cancellous bone particles; treating the bone particles with hot water and an organic solvent; repeating the treating step at least once; drying the bone particles; and heating the bone particles at 500° C. to 620° C. for 10 to 50 hours.
1. A method of preparing carbonate apatite from natural bone, the method consisting of:
providing cancellous bone particles;
treating the bone particles with hot water and an organic alcohol to remove lipids therefrom;
repeating the treating step at least once;
drying the treated bone particles; and
heating the dried treated bone particles at 500° C. to 620° C. for 10 to 50 hours.
2. The method of claim 1 , wherein the size of the bone particles is 2 mm to 15 mm.
3. The method of claim 2 , wherein the size of the bone particles is 5 mm to 10 mm.
4. The method of claim 1 , wherein the bone particles are first treated with hot water, and then extracted with an organic alcohol at room temperature.
5. The method of claim 1 , wherein the organic alcohol is isopropanol.
6. The method of claim 1 , wherein the organic alcohol is ethanol.
7. The method of claim 1 , wherein the bone particles are treated with hot water for 2 to 8 hours.
8. The method of claim 1 , wherein the bone particles are treated with an organic alcohol for 16 to 24 hours.
9. The method of claim 8 , wherein the organic alcohol is isopropanol.
10. The method of claim 1 , wherein the temperature of the hot water is 80° C. to 100° C.
11. The method of claim 1 , wherein the bone particles are heated at 590° C. to 605° C.
12. The method of claim 11 , wherein the bone particles are heated for 22 to 33 hours.
13. The method of claim 1 , wherein the bone particles are heated for 20 to 40 hours.
14. The method of claim 13 , wherein the bone particles are heated for 22 to 33 hours.
15. The method of claim 1 , wherein the heating step is carried out in a furnace.
16. A method of preparing carbonate apatite from natural bone, the method consisting of:
obtaining cancellous bone particles by first removing adhering tissues, cartilage tissues and the cortical portion from a bone, and then grinding the cancellous portion of the bone into particles;
treating the bone particles with hot water and an organic alcohol to remove lipids therefrom;
repeating the treating step at least once;
drying the treated bone particles; and
heating the dried treated bone particles at 500° C. to 620° C. for 10 to 50 hours.