Biochip and method for manufacturing the same
View Patent ↗A biochip and a method for manufacturing the same are provided. The biochip can increase a surface area on which biomaterials can be immobilized and improve efficiency of reaction. The biochip includes: a substrate; a coating layer formed on the substrate; and a nanoparticle immobilized on the substrate by the coating layer, a material to be bound to a target material being attached on a surface of the nanoparticle.
1. A biochip, comprising:
a substrate;
a silica coating layer disposed on the substrate and comprising silica particles; nanoparticles attached to the substrate by the silica coating layer; and
a target binding material attached to the nanoparticles,
wherein the silica coating layer is formed by coating the substrate with silica sol comprising silica particles that each have a diameter of 5 to 50 nm.
2. The biochip of claim 1 , wherein the substrate is formed of at least one of silicon, glass, metal, inorganic material, polymer, and high molecular weight material.
3. The biochip of claim 1 , wherein the substrate comprises one or more selected from the group consisting of poly(dimethylsiloxane) (PDMS), poly(methyl methacrylate) (PMMA), cyclic olefin copolymer (COC), polycarbonate (PC), polyamide (PA), polyethylene (PE), polypropylene (PP), polyphenylene ether (PPE), polystyrene (PS), polyoxymethylene (POM), polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), polyvinyl chloride (PVC), polyvinylidene fluoride (PVDF), polybutylene terephthalate (PBT), fluorinated ethylene propylene (FEP), and perfluor alkoxyalkane (PFA).
4. The biochip of claim 1 , wherein the silica particles are smaller than the nanoparticles.
5. The biochip of claim 1 , wherein the nanoparticles comprise silica nanoparticles.
6. The biochip of claim 1 , wherein the nanoparticles each have a diameter of 100 to 800 nm.
7. The biochip of claim 1 , further comprising a biomaterial specifically bound to the target binding material,
wherein the biomaterial is at least one of an antibody, an enzyme, a protein, DNA, RNA, PNA, a nucleic acid, and a oligopeptide.
8. A method for manufacturing a biochip, comprising:
coating a substrate with a silica sol comprising silica particles that each have a diameter of 5 to 50 nm to form a silica coating layer;
attaching nanoparticles to the silica coating layer; and
attaching a target binding material to the nanoparticles.
9. The method of claim 8 , wherein the coating of the substrate comprises drop-casting, a dip-coating, and syringe pump injection.
10. The method of claim 8 , further comprising drying the silica coating layer before attaching the nanoparticles.
11. The method of claim 8 , wherein the attaching of the nanoparticles comprises positioning the nanoparticles using a pipette or an ink-jet printing method.
12. The method of claim 8 , wherein the substrate comprises one or more selected from the group consisting of poly(dimethylsiloxane) (PDMS), poly(methyl methacrylate) (PMMA), cyclic olefin copolymer (COC), polycarbonate (PC), polyamide (PA), polyethylene (PE), polypropylene (PP), polyphenylene ether (PPE), polystyrene (PS), polyoxymethylene (POM), polyetheretherketone (PEEK), polytetrafluoroethylene (PTFE), polyvinyl chloride (PVC), polyvinylidene fluoride (PVDF), polybutylene terephthalate (PBT), fluorinated ethylene propylene (FEP), and perfluor alkoxyalkane (PFA).
13. The method of claim 8 , wherein the nanoparticles comprise silica nanoparticles that each have a diameter of 100 to 800 nm.
14. The method of claim 8 , further comprising reacting the target binding material with a biomaterial.
15. The method of claim 14 , wherein the biomaterial comprises at least one of an antibody, an enzyme, a protein, DNA, RNA, PNA, a nucleic acid, and an oligopeptide, capable of specifically binding to the target binding material.