IP Library › Patent Application 13255878
Patent Application
App. No. 13/255,878

ENGINEERING SALT TOLERANCE IN PHOTOSYNTHETIC MICROORGANISMS

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Patent No.
US None
App. No.
13/255,878
Abstract

Provided herein are compositions and methods for engineering salt tolerance and producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to be salt tolerant as compared to an unmodified organism and to produce useful products. The methods and compositions of the disclosure are useful in many therapeutic and industrial applications.

Claims (24)

1 - 415 . (canceled)

416 . An isolated polynucleotide capable of transforming a photosynthetic organism, wherein the polynucleotide comprises a nucleic acid sequence of SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60; or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60; wherein the nucleic acid sequence encodes for a protein that when expressed results in a salt tolerant photosynthetic organism as compared to a photosynthetic organism that is not transformed by the isolated polynucleotide.

417 . The isolated polynucleotide of claim 416 , wherein the photosynthetic organism is an alga.

418 . The isolated polynucleotide of claim 417 , wherein the alga is an alga of the genus Nannochloropsis, Chlamydomonas, Scenedesmus , or Dunaliella.

419 . The isolated polynucleotide of claim 416 , wherein the photosynthetic organism is a cyanobacteria.

420 . The isolated polynucleotide of claim 419 , wherein the cyanobacteria is a cyanobacteria from the genus Spirulina.

421 . The isolated polynucleotide of claim 416 , wherein the polynucleotide further comprises a second nucleic acid sequence.

422 . The isolated polynucleotide of claim 421 , wherein the second nucleic acid sequence encodes for a chaperonin, an antioxidant, a biodegradative enzyme, exo-β-glucanase, endo-β-glucanase, β-glucosidase, endoxylanase, lignase, a flocculating moiety, a botryococcene synthase, a limonene synthase, a 1,8 cineole synthase, a α-pinene synthase, a camphene synthase, a (+)-sabinene synthase, a myrcene synthase, an abietadiene synthase, a taxadiene synthase, a farnesyl pyrophosphate synthase, an amorphadiene synthase, a (E)-α-bisabolene synthase, a diapophytoene synthase, a diapophytoene desaturase, a transporter, a protein that regulates the expression of a transporter, a protein that confers salt tolerance to an organism, a BBC protein or a functional homolog of a BBC protein, a SCSR protein or a functional homolog of a SCSR protein, a therapeutic protein, a nutritional protein, an industrial enzyme, a protein that participates in or promotes the synthesis of at least one nutritional product, therapeutic product, commercial product, or fuel product, or a protein that facilitates the isolation of at least one nutritional product, therapeutic product, commercial product, or fuel product.

423 . The isolated polynucleotide of claim 416 , wherein the nucleic acid sequence and/or second nucleic acid sequence are operably linked to a promoter.

424 . The isolated polynucleotide of claim 423 , wherein the promoter is an RBCS promoter, an LHCP promoter, a tubulin promoter, a pSAD promoter, a chimeric promoter, HSP70A/rbcS2, a constitutive promoter, an inducible promoter, a NIT1 promoter, a CYC6 promoter, or a CA1 promoter.

425 . A vector comprising the isolated polynucleotide of claim 423 .

426 . A photosynthetic organism transformed with the polynucleotide of claim 423 .

427 . An isolated photosynthetic organism transformed with an exogenous polynucleotide, wherein the exogenous polynucleotide comprises at least one nucleic acid sequence encoding a protein that when expressed in the photosynthetic organism, results in the photosynthetic organism becoming a more salt tolerant photosynthetic organism as compared to a photosynthetic organism that is not transformed by the exogenous polynucleotide, and wherein the at least one nucleic sequence is SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60; or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14. SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60.

428 . The isolated photosynthetic organism of claim 427 , wherein the photosynthetic organism is an alga.

429 . The isolated photosynthetic organism of claim 428 , wherein the alga is an alga of the genus Nannochloropsis, Chlamydomonas, Scenedesmus , or Dunaliella.

430 . The isolated photosynthetic organism of claim 427 , wherein the photosynthetic organism is a cyanobacteria.

431 . The isolated photosynthetic organism of claim 430 , wherein the cyanobacteria is a cyanobacteria from the genus Spirulina.

432 . The isolated photosynthetic organism of claim 427 , wherein the photosynthetic organism is cultured or grown in a media comprising NaCl.

433 . A method for increasing salt tolerance of a photosynthetic organism comprising, (a) transforming the photosynthetic organism with an exogenous polynucleotide sequence comprising a nucleic acid sequence, wherein the nucleic acid sequence encodes a protein that when expressed in the photosynthetic organism, results in increased salt tolerance of the photosynthetic organism as compared to a photosynthetic organism that is not transformed by the exogenous polynucleotide sequence, and wherein the nucleic acid sequence is SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60; or a nucleic acid sequence comprising at least 80%, at least 85%, at least 90%, at least 95%, at least 98%, or at least 99% sequence identity to a nucleic acid sequence of SEQ ID NO: 55, SEQ ID NO:53, SEQ ID NO: 39, SEQ ID NO: 37, SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO:41, SEQ ID NO: 43, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, SEQ ID NO: 57, SEQ ID NO: 59, or SEQ ID NO: 60.

434 . The method of claim 433 , wherein the photosynthetic organism is arm alga.

435 . The method of claim 434 , wherein the alga is an alga of the genus Nannochloropsis, Chlamydomonas, Scenedesmus , or Dunaliella.

436 . The method of claim 433 , wherein the photosynthetic organism is a cyanobacteria.

437 . The method of claim 436 , wherein the cyanobacteria is a cyanobacteria from the genus Spirulina.

438 . The method of claim 433 , wherein the exogenous polynucleotide sequence comprises a second nucleic acid sequence.

Assignments (5)
RELEASE OF SECURITY INTEREST IN PATENT COLLATERAL AT REEL/FRAME NO. 30157/0052 Recorded Dec 15, 2015
From: THE WELLCOME TRUST LIMITED
To: SAPPHIRE ENERGY, INC.
Reel/Frame 037300/0279 →
SECURITY AGREEMENT Recorded Apr 5, 2013
From: SAPPHIRE ENERGY, INC.
To: THE WELLCOME TRUST LIMITED
Reel/Frame 030157/0052 →
RELEASE OF SECURITY INTEREST Recorded Apr 5, 2013
From: SILICON VALLEY BANK
To: SAPPHIRE ENERGY, INC.
Reel/Frame 030162/0247 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 16, 2011
From: MENDEZ, MICHAEL; FANG, SU-CHIUNG; RICHARD, STEPHANE
To: SAPPHIRE ENERGY, INC.
Reel/Frame 027401/0795 →
SECURITY AGREEMENT Recorded Oct 25, 2011
From: SAPPHIRE ENERGY, INC.
To: SILICON VALLEY BANK
Reel/Frame 027119/0886 →