Tissue engineering supports and methods therefor
View Patent ↗The present disclosure relates to supports and scaffolds for cell and tissue engineering. The supports disclosed herein are composed of a thermally responsive material, containing pillars, that is coated with an acrylic polymer, thereby imparting an amphipathic matrix foundation. When exposed to a change in temperature, the coated support reacts by facilitating or repelling hydromolecular interactions. Further disclosed herein are methods for making hydrogels that can support tissue growth.
1. A support for tissue engineering comprising:
a thermoconductive scaffold with one or more pillars, wherein the one or more pillars have a length from about 0.5 cm to 10 cm and a diameter from about 0.5 mm-5 mm; and
a thermoresponsive polymer coating the one or more pillars, wherein the thermoresponsive polymer is capable of transitioning from a hydrophobic to a hydrophilic state in response to a change in temperature.
2. The support of claim 1 , wherein the thermoconductive scaffold is made of a material selected from the group consisting of copper, silver, zinc, magnesium, iron, gold, aluminum, aluminum nitride, aluminum oxide, brass, cobalt, graphite, and beryllium, or any combination thereof.
3. The support of claim 1 , wherein the thermoconductive scaffold is made of copper.
4. The support of claim 1 , wherein the one or more pillars are positioned throughout the thermoconductive scaffold in angled, straight, slanted, tapered, polygonal, rectangular, square, circular, curved, diagonal, random, concentric, patterned, perimetric, polygonal, diamond, hexagonal, or triangular configurations, or any combination thereof.
5. The support of claim 1 , wherein the thermoresponsive polymer is PIPAAm.
6. The support of claim 5 , wherein the PIPAAm is hydrophobic at temperatures above 32° C. and wherein the PIPAAm is hydrophilic at 32° C. or below.
7. A kit for providing tissue engineering support comprising:
a thermoconductive scaffold with one or more pillars, wherein the one or more pillars are coated in a thermoresponsive polymer, and wherein the length of the one or more pillars is from about 0.5 cm to 10 cm and the diameter is from about 0.5 mm-5 mm;
one or more hydrogel components; and
instructions for making the support.