Yeast Strain and Method for Using the Same to Produce Nicotinamide Riboside

1 . An isolated fungal strain deficient in nicotinamide riboside import and salvage.

2 . The fungal strain of claim 1 , wherein said strain does not express Nicotinamide Riboside Kinase 1 (Nrk1), Uridine Hydrolase 1 (Urh1), Purine Nucleoside Phosphorylase (Pnp1), and Nicotinamide Riboside Transporter 1 (Nrt1).

3 . The fungal strain of claim 1 , wherein said strain secretes at least 8 mg/L nicotinamide riboside.

4 . The fungal strain of claim 1 , wherein said fungus is selected from the group consisting of Saccharomyces, Schizosaccharomyces, Kluveromyres, Aspergillus and Pichia.

5 . The fungal strain of claim 1 , wherein said fungus is Saccharomyces cerevisiae.

6 . A method for producing nicotinamide riboside comprising culturing the fungal strain of claim 1 in culture medium and recovering nicotinamide riboside from the medium thereby producing nicotinamide riboside.

7 . The method of claim 6 , wherein the culture medium comprises nicotinic acid or nicotinamide.

8 . The method of claim 6 , wherein the fungal strain is cultured to an optical density of at least 3.

9 . The method of claim 6 , wherein the nicotinamide riboside is recovered by solubilizing nicotinamide riboside from the medium with methanol and subjecting the nicotinamide riboside to column chromatography.

10 . A method for producing a nicotinamide riboside-supplemented food product comprising providing a fermentable substrate and fermenting the fermentable substrate in the presence of the fungal strain of claim 1 thereby producing a nicotinamide riboside supplemented food product.

11 . A nicotinamide riboside supplemented food product fermented in the presence of the fungal strain of claim 1 .

12 . The product of claim 11 , wherein said food product is wine, beer, cider, kvass, root beer, soy sauce or bread.