Microalgal polysaccharide compositions
View Patent ↗Provided herein are microalgal polysaccharide compositions and. Also provided are methods of using polysaccharides for applications such as topical personal care products, cosmetics, and wrinkle reduction compositions. The invention also provides novel microalgal compositions useful for improving the health and appearance of skin. The invention also includes insoluble polysaccharide particles for application to human skin.
1. A composition comprising isolated exopolysaccharide from microalgae of the species Parachlorella kessleri or Parachlorella beijerinckii , wherein the monosaccharide content of the exopolysaccharide comprises 15-55 mole percent rhamnose, 3-30 mole percent xylose, 1-25 mole percent mannose, 1-45 mole percent galactose, 0.5-10 mole percent glucose, and 0.1-15 percent glucuronic acid, and wherein the composition is prepared by drying and annealing at a temperature of between about 125° C. and about 155° C. so as to form particles that are partially soluble in water and increase in volume at least two-fold upon contact with water compared to the anhydrous volume of the exopolysaccharide particles.
2. The composition of claim 1 , wherein the exopolysaccharide is milled to provide exopolysaccharide particles having an average particle size of between about 400 microns and about 0.1 microns.
3. The composition of claim 2 , wherein the average particle size of the exopolysaccharide particles is between about 50 microns and about 200 microns.
4. The composition of claim 2 , wherein the exopolysaccharide particles resuspended in water have a viscosity of between about 500 cP and about 1000 cP.
5. The composition of claim 2 , wherein the exopolysaccharide particles are from Parachlorella kessleri.
6. The composition of claim 2 , wherein the exopolysaccharide particles are from Parachlorella beijerinckii.
7. The composition of claim 2 , wherein the exopolysaccharide particles are formulated with at least one excipient suitable for topical administration.
8. A composition comprising an isolated polysaccharide from a microalgae having at least 95% 23S rRNA genomic sequence identity to SEQ ID NO:3 or SEQ ID NO: 4.
9. A composition comprising isolated exopolysaccharide from microalgae of the species Parachlorella kessleri or Parachlorella beijerinckii wherein the monosaccharide content of the exopolysaccharide comprises 15-55 mole percent rhamnose, 3-30 mole percent xylose, 1-25 mole percent mannose, 1-45 mole percent galactose, 0.5-10 mole percent glucose, and 0.1-15 percent glucuronic acid, and wherein the composition is prepared by drying and annealing at a temperature of between about 125° C. and about 155° C. so as to form particles that are partially soluble in water and increase in volume at least 2-fold upon contact with water compared to the anhydrous volume of the exopolysaccharide particles.