Biosensor system and method for measuring concentration of analyte

1. A biosensor system, with which the concentration of an analyte in a liquid sample is measured using a redox enzyme and a potassium ferricyanide as an electron-transfer mediator, said biosensor system comprising:

a sensor chip having a capillary into which a liquid sample is introduced, a plurality of electrodes disposed within the capillary, and a reagent layer that is disposed within the capillary and includes the electron-transfer mediator;

the capillary having a height less than the maximum value of the sum of the diffusion distance of the electron-transfer mediator and the diffusion distance of the analyte at an upper temperature limit for which the biosensor system is functional;

the plurality of electrodes including a working electrode and a counter electrode; and

the plurality of electrodes are disposed so as to be opposite each other in a horizontal plane;

a voltage applicator that

applies a measurement voltage to the working electrode and the counter electrode,

applies a second voltage of positive polarity to the working electrode and the counter electrode prior to the application of the measurement voltage, so that the effect of the temperature of the liquid sample on the measurement results of the concentration measurement section will be diminished, and

applies a first voltage to the working electrode and the counter electrode prior to the application of the second voltage,

wherein the second voltage is positive with respect to a ground used when applying the measurement voltage, and

wherein the second voltage is lower than the measurement voltage;

a concentration measurement section that measures the concentration of the analyte on the basis of the value of the current flowing through the liquid sample during the measurement voltage application; and

wherein the height of the capillary of the sensor chip is the distance from the working electrode to an opposite face from the working electrode, and the height of the capillary of the sensor chip is between 49 and 90 μm.

2. The biosensor system according to claim 1 ,

wherein the height of the capillary of the sensor chip is less than the maximum value of the sum of the diffusion distance of the electron-transfer mediator and the diffusion distance of the analyte, found from a lower temperature limit for which biosensor system is functional.

3. The biosensor system according to claim 1 ,

wherein the voltage applicator accumulates electrons in the electron-transfer mediator by applying the second voltage.

4. The biosensor system according to claim 1 ,

wherein the voltage applicator applies an open circuit voltage as the second voltage.

5. The biosensor system according to claim 1 ,

wherein the concentration measurement section has a calibration curve or table that correlates the current value and the analyte concentration, and calculates the analyte concentration on the basis of the same calibration curve or table even if the temperature of the liquid sample should fluctuate.

6. The biosensor system according to claim 1 ,

wherein the concentration measurement section measures the analyte concentration on the basis of the current value at a point when no more than 10 seconds have elapsed since the start of the application of the second voltage.

7. The biosensor system according to claim 1 ,

wherein the electrodes are disposed on two faces that are mutually opposite in the height direction of the capillary.

8. The biosensor system according to claim 1 ,

wherein the second voltage is smaller than the measurement voltage, and

the first voltage is bigger than the second voltage.

9. The biosensor system according to claim 1 ,

wherein the height of the capillary of the sensor chip is between 49 and 59 μm.