GAS-FREE FLUID CHAMBER

1 . A fluid chamber ( 1 ) being in communication with,

a first channel ( 2 ) suitable for functioning as an inlet for fluids into said fluid chamber;

a second channel ( 3 ) suitable for functioning as an outlet for fluids out of the fluid chamber;

wherein at least one protrusion ( 4 ) projects into the fluid chamber,

and wherein said protrusion ( 4 ) is positioned at the locations where the second channel ( 3 ) is connected to the fluid chamber.

2 . A fluid chamber ( 1 ) wherein the surface of said protrusion ( 4 ) inside the fluid chamber ( 1 ) is smooth.

3 . A fluid chamber ( 1 ) according to claim 2 , wherein the protrusion ( 4 ) is of circular or elliptical shape.

4 . A fluid chamber ( 1 ) according to claim 1 , wherein the fluid chamber is of cylindrical form with a circular or elliptical cross-sectional shape ( 5 ), when viewed from above; and

wherein the first channel ( 2 ) and the second channel ( 3 ) are connected to the side walls of fluid chamber of cylindrical form.

5 . A fluid chamber according to claim 1 , wherein the diameter ( 6 ) of the fluid chamber ( 1 ) is in the range of about 100 μm to about 10 cm and wherein the height of the fluid chamber is in the range of about 100 μm to about 1 cm.

6 . A fluid chamber according to claim 1 , wherein the diameter ( 7 ) of the protrusion ( 4 ) of circular or elliptical shape is smaller than the diameter ( 6 ) of the fluid chamber ( 1 ) by a factor of equal to or at least about 10.

7 . A fluid chamber according to claim 1 , wherein the diameter ( 7 ) of the protrusion ( 4 ) of circular or elliptical shape is in the range of about 10 μm to about 1 cm.

8 . A fluid chamber according to claim 1 , wherein the fluid chamber ( 1 ) is configured such that it is suitable for performing polymerase chain reactions in the fluid chamber.

9 . A fluid chamber according to claim 1 , wherein means for controlling the temperature within the fluid chamber are in communication with the fluid chamber.

10 . A fluid chamber according to claim 1 , wherein the fluid chamber comprises at least one transparent section.

11 . A fluid chamber according to claim 1 , wherein the fluid chamber is made from polypropylene.

12 . Use of a fluid chamber according to claim 1 for gas-free filling with a liquid.

13 . Method of completely filling a fluid chamber with a liquid comprising at least the following steps:

a. Providing a fluid chamber according to claim 1 ;

b. Introducing a liquid into the first channel ( 2 ) of a fluid chamber according to claim 1 .

14 . Device comprising a fluid chamber of claim 1 .

15 . Device of claim 14 wherein the device is a cartridge.