Methods and Apparatus for Analyzing Samples and Collecting Sample Fractions
Methods and apparatus for analyzing a sample using at least one detector are disclosed.
1 . A method of analyzing a sample, said method comprising the step of:
(a) generating a signal from one or more detectors in a liquid chromatography system, the signal comprising a detection response component from at least one detector; and
(b) collecting a new sample fraction in a fraction collector in response to a change in the signal;
wherein amplitude of the signal is modified by the liquid chromatography system.
2 . The method of claim 1 , wherein the amplitude of the signal is modified by electronic or digital means.
3 . The method of claim 1 , wherein the amplitude of the signal is modified by changing the gain of the signal using a component of the chromatography system.
4 . The method of claim 1 , wherein the amplitude of the signal is modified by computer software or a computer readable medium.
5 . The method of claim 1 , wherein the amplitude of the signal is modified by optical means.
6 . The method of claim 1 , wherein the amplitude of the signal is modified by a light source in the one or more of the detectors of the chromatography system.
7 . The method of claim 1 , wherein the amplitude of the signal is modified by using a different light source in each of the one or more of the detectors of the chromatography system.
8 . The method of claim 1 , wherein the amplitude of the signal is modified by changing the intensity of a light source in the one or more of the detectors of the chromatography system.
9 . The method of claim 1 , wherein the amplitude of the signal is modified by using multiple light sources in each of the one or more of the detectors of the chromatography system.
10 . The method of claim 1 , wherein the amplitude of the signal is modified by fluidic means.
11 . The method of claim 1 , wherein the amplitude of the signal is modified by changing the amount of sample transferred to the one or more of the detectors of the chromatography system.
12 - 60 . (canceled)
61 . An apparatus for analyzing a sample, said apparatus comprising:
(a) system hardware operatively adapted to generate a signal from one or more detectors in a liquid chromatography system, the signal comprising a detection response component from one or more detectors; and
(b) a fraction collector operatively adapted to collect a new sample fraction in response to a change in the signal;
wherein the liquid chromatography system is operatively adapted to modify amplitude of the signal.
62 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by electronic or digital means.
63 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by a component of the chromatography system being operatively adapted to change the gain of the signal.
64 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by computer software or a computer readable medium.
65 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by optical means.
66 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by a light source in the one or more of the detectors of the chromatography system.
67 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by the chromatography system being operatively adapted to use a different light source in each of the one or more of the detectors.
68 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by the chromatography system being operatively adapted to vary the intensity of a light source in the one or more of the detectors.
69 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by the chromatography system being operatively adapted to use multiple light sources in each of the one or more of the detectors.
70 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by fluidic means.
71 . The apparatus of claim 61 , wherein the amplitude of the signal is modified by the chromatography system being operatively adapted to vary the amount of sample transferred to the one or more of the detectors.
72 - 114 . (canceled)