Systems and methods for sample collection

1. A system for sample collection, the system comprising:

a tube assembly comprising:

a first tube having first and second ends, the first end of the first tube having an outer diameter and having a first tube opening therein;

a second tube having a first end and a second end, the first end of the second tube having a second tube opening therein and the second end of the second tube having a third tube opening therein, the second tube opening having an inner diameter greater than the outer diameter of the first end of the first tube;

a securing mechanism operable to secure the first tube and the second tube such that the first end of the second tube extends beyond the first end of the first tube, with the first end of the first tube inside the first end of the second tube creating an interstitial space about the first tube at the first end of the second tube; and

a seal, wherein when the seal is disposed against the first end of the second tube to form a barrier, wherein a biological sample can pass from the first end of the first tube by way of the interstitial space into the third tube opening of the second end of the second tube;

a sample source fluidly coupled to the second end of the first tube; and

a collection vessel comprising a collection medium, wherein the collection medium is capable of binding biological samples, and wherein the collection vessel is positioned to receive a fluid from the tube assembly.

2. The system of claim 1 , further comprising a controller coupled to the collection vessel and the tube assembly, the controller operable to change the time of receipt of the fluid or the quantity of the fluid received.

3. The system of claim 1 , wherein the collection medium comprises a nitrocellulose material.

4. The system of claim 1 , wherein when the seal is disposed against the first end of the second tube to form a barrier, wherein a biological sample can pass from the second end of the second tube by way of the interstitial space into the first tube opening of the first end of the first tube.

5. The system of claim 1 , wherein the securing mechanism comprises a threaded hub.

6. The system of claim 5 , wherein the threaded hub is sized and shaped to form a cavity for receipt of a portion of the first tube and the second tube.

7. The system of claim 6 , wherein the securing mechanism further comprises an adhesive within the cavity of the threaded hub to fixedly secure the first tube and second tube.

8. The system of claim 5 , wherein the first end of the second tube extends a fixed distance beyond the first end of the first tube, the distance selected from the group consisting of about 0.3 mm to about 1.6 mm, about 0.5 mm to about 1.3 mm, about 0.6 mm to about 1.0 mm, and about 0.8 mm.

9. A method of sample collection, the method comprising the steps of:

introducing a biological sample into a tube assembly of a system of sample collection from a sample source, the system comprising:

the tube assembly comprising:

a first tube having first and second ends, the first end of the first tube having an outer diameter and having a first tube opening therein;

a second tube having a first end and a second end, the first end of the second tube having a second tube opening therein and the second end of the second tube having a third tube opening therein, the second tube opening having an inner diameter greater than the outer diameter of the first end of the first tube;

a securing mechanism operable to secure the first tube and the second tube such that the first end of the second tube extends beyond the first end of the first tube, with the first end of the first tube inside the first end of the second tube creating an interstitial space about the first tube at the first end of the second tube;

a seal, wherein when the seal is disposed against the first end of the second tube to form a barrier, wherein a biological sample can pass from the first end of the first tube by way of the interstitial space into the third tube opening of the second end of the second tube;

the sample source fluidly coupled to the second end of the first tube; and

a collection vessel comprising a collection medium, wherein the collection medium is capable of binding biological samples, and wherein the collection vessel is positioned to receive a fluid from the tube assembly; and

depositing the biological sample from the tube assembly on the collection media;

wherein the biological sample deposited on the collection media is undiluted.

10. The method of claim 9 , wherein the collection medium comprises a nitrocellulose material.

11. The method of claim 9 , wherein the securing mechanism comprises a threaded hub.

12. The method of claim 11 , wherein the threaded hub is sized and shaped to form a cavity for receipt of a portion of the first tube and the second tube.

13. The method of claim 11 , wherein the securing mechanism further comprises an adhesive within the cavity of the threaded hub to fixedly secure the first tube and second tube.

14. The method of claim 9 , wherein the first end of the second tube extends a fixed distance beyond the first end of the first tube, the distance selected from the group consisting of about 0.3 mm to about 1.6 mm, about 0.5 mm to about 1.3 mm, about 0.6 mm to about 1.0 mm, and about 0.8 mm.

15. The system of claim 1 , wherein the seal comprises an elastomeric seal material.