Methods for making plants resistant to fungal pathogens
This invention relates to polynucleotide sequences encoding genes that can confer resistance to the plant pathogen Colletotrichum , which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum , and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the genes from corn lines carrying said genes into other corn lines that do not carry said genes, in order to make them resistant to Colletotrichum and resistant to stalk rot.
1. A process of identifying a corn plant that displays enhanced resistance to Colletotrichum infection, the process comprising detecting in the corn plant the presence or absence of at least one marker at the Rcg1 locus, wherein said at least one marker is on or within the polynucleotide sequence of SEQ ID NO: 257 and selecting the corn plant in which said at least one marker is present.
2. The process of claim 1 , wherein the at least one marker is on or within the Rcg1b coding sequence.
3. The process of claim 1 , wherein the Rcg1b coding sequence comprises a nucleotide sequence encoding a polypeptide, wherein the polypeptide has an amino acid sequence of at least 95% identity when compared to SEQ ID NO: 246 based on the Needleman-Wunsch alignment algorithm and wherein the polypeptide confers Colletotrichum resistance.
4. The process of claim 1 , wherein the at least one marker detects a polynucleotide sequence encoding an mRNA sequence derived from the Rcg1b mRNA transcript and unique to Rcg1b.
5. The process of claim 1 , further comprising a second marker on or within the Rcg1 coding sequence.
6. The process of claim 5 , wherein the Rcg1 coding sequence comprises a nucleotide sequence encoding the polypeptide set forth in SEQ ID NO:3.
7. The process of claim 1 , further comprising a second marker that detects a polynucleotide sequence encoding an mRNA sequence derived from the Rcg1b mRNA transcript and unique to Rcg1.
8. The process of claim 1 further comprising a second marker selected from the group consisting of:
UMC2041, AY112127, UMC1086, AY110631, UMC2285, MZA8136, MZA6064, NPI270, NPI300C, PHP20071, CDO127a, RGPI102, UAZ122, BNL17.05, MZA11455, MZA15842, MZA11123, MZA2591, PHI093, MZA1215, MZA1216, MZA3434, CL12681 1, NPI444, UMC15a, MZA8761, CSU166a, CDO365, CSU1038b, CSU1073b, CSU597a, RGPG111, UMN433, PHP20562, C2, NPI910, CSU178a, CSU202, TDA44, MZA1851, UMC1051, MZA11394, PCO136722, UMC2187, NPI410, PSR109B, UMC1371, UMC1842, UMC1856, AY109980, UMC1132, NFD106, AY105971, AY110989, ENSI002A, RZ596B, BNL23A, BNL29, UMC2200, UMC1086, AY110631, UMC2285, MZA8136, MZA6064, NPI270, NPI300C, PHP20071, CDO127a, RGPI102, UAZ122, BNL17.05, MZA11455, MZA15842, MZA11123, MZA2591, PHI093, MZA1215, MZA1216, MZA3434, CL12681 1, NPI444, UMC15a, MZA8761, CSU166a, CDO365, CSU1038b, CSU1073b, CSU597a, RGPG111, UMN433, PHP20562, C2, NPI910, CSU178a, CSU202, TDA44, MZA1851, UMC1051, MZA11394, PCO136722, UMC2187, NPI410, PSR109B, UMC1371, UMC1842, UMC1856, AY109980, UMC1132, NFD106, AY105971, AY110989, ENSI002A, RZ596B, BNL23A, BNL29, UMC2200, UMC2285, MZA8136, MZA6064, NPI270, NPI300C, PHP20071, CDO127a, RGPI102, UAZ122, BNL17.05, MZA11455, MZA15842, MZA11123, MZA2591, PHI093, MZA1215, MZA1216, MZA3434, CL12681 1, NP1444, UMC15a, MZA8761, CSU166a, CDO365, CSU1038b, CSU1073b, CSU597a, RGPG111, UMN433, PHP20562, C2, NPI910, CSU178a, CSU202, TDA44, MZA1851, UMC1051, MZA11394, PCO136722, UMC2187, MZA8136, MZA6064, NPI270, NPI300C, PHP20071, CDO127a, RGPI102, UAZ122, BNL17.05, MZA11455, MZA15842, MZA11123, MZA2591, PHI093, MZA1215, MZA1216, MZA3434, CL12681 1, and NP1444.