IP Library › Granted Patent US 9,018,014
Granted Patent B2
US 9,018,014 · App. 13/274,643 · Granted Apr 28, 2015

Vectors for directional cloning

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Quick Facts
Patent No.
US 9,018,014
App. No.
13/274,643
Granted
Apr 28, 2015
Kind
B2
Abstract

The invention provides vectors and methods for directional cloning.

Claims (2)

1. A method comprising introducing a vector comprising a first promoter operably linked to a nucleic acid fragment encoding a barnase which lacks a secretory domain into a recombinant host cell which expresses barstar from a second promoter which is constitutively expressed in prokaryotic cells, wherein the barnase is lethal to the recombinant host cell when expressed in the absence of barstar, wherein the host cell is a prokaryotic cell.

2. The method of claim 1 , wherein the first promoter comprises a promoter expressed in a prokaryotic cell.

Assignments (1)
SECURITY INTEREST Recorded Apr 3, 2019
From: PROMEGA CORPORATION; PROMEGA BIOSCIENCES, LLC; TERSO SOLUTIONS, INC.; ORION SEVEN, LLC; PROMEGA AVIATION LLC
To: JPMORGAN CHASE BANK, N.A., AS COLLATERAL AGENT
Reel/Frame 048790/0259 →