Mephedrone detection
View Patent ↗The invention relates to an immunoassay method and kit for the detection and/or the determination of mephedrone, mephedrone metabolites and related compounds. The invention is underpinned by a novel antibody, derived from a novel immunogen, that is sensitive and binds to mephedrone, mephedrone metabolites and related compounds.
1. A method of detecting or determining at least one molecule of Structure 1
wherein B is selected from the group consisting of H and CH 3 , and D is selected from the group consisting of H, CH 3 , —O—CH 3 and F, in an in vitro sample taken from a patient, the method comprising
a) contacting the sample with an antibody and a conjugate, the antibody having a cross-reactivity of 100% to mephedrone, and having a cross reactivity of greater than 10% to an epitope of each of cathinone and methcathinone, the antibody being raised against an immunogen of the structure
wherein,
accm is an antigenicity conferring carrier material selected from the group consisting of bovine serum albumin (BSA), egg ovalbumin, bovine gamma globulin, bovine thyroglobulin (BTG), and keyhole limpet haemocyanin (KLH);
X is either carbonyl or amino;
Y is —Z—(A) n — in which Z is a C 1-5 , substituted or unsubstituted straight chain alkylene or arylene moiety,
A is O, NH, S, ester, thioester, or amide, and
n=0 or 1 wherein, when A is present, it is linked to the phenyl ring;
b) measuring the conjugate; and
c) deducing from a calibrator, the presence of or amount of the at least one molecule of Structure 1.
2. The method of claim 1 in which the antibody has a cross-reactivity of greater than 15% to an epitope of each of cathinone and methcathinone.
3. The method of claim 1 in which the at least one molecule of Structure 1 is selected from at least one of mephedrone, methcathinone and cathinone.
4. The method of claim 3 in which the antibody has a cross-reactivity of greater than 15% to an epitope of each of cathinone and methcathinone.