Process for treatment of residual nucleic acids present on the surface of laboratory consumables
View Patent ↗Process for treatment of residual nucleic acids present on the surface of laboratory consumables. The process combines two treatment phases: (1) treatment with ethylene oxide in gaseous phase; then (2) treating the surface with hydrogen peroxide in liquid phase or in gaseous phase. The effect of this treatment is the avoidance of the amplification of residual nucleic acids, in particular during PCR or TMA reactions.
1. A method of eliminating amplifiable residual nucleic acids present on the surface of a consumable, comprising:
i) Treating the surface of said consumable with ethylene oxide in gaseous phase; then
ii) Treating said surface with hydrogen peroxide in liquid phase or in gaseous phase.
2. A method according to claim 1 , wherein the ethylene oxide at step i) is used with a concentration of between 70% and 90% (v/v).
3. A consumable treated according to the method of claim 2 , wherein it is free of amplifiable residual nucleic acids.
4. A method according to claim 1 or 2 , wherein the hydrogen peroxide is in liquid phase at a concentration between 25% and 60% (v/v).
5. A method according to claim 2 or 4 , wherein the hydrogen peroxide, in liquid phase, is in contact with the surface to be decontaminated for a period of at least 40 minutes.
6. A method according to claim 4 , wherein the hydrogen peroxide in liquid phase is heated to a temperature between 50° C. and 70° C.
7. A method according to claim 1 , wherein the treatment with hydrogen peroxide is carried out in gaseous phase.
8. A method according to claim 7 , wherein the hydrogen peroxide is vaporized, then brought to a concentration greater than 30%.
9. A method according to claim 8 , wherein the hydrogen peroxide is passed into the plasma state by application of an electric field at a temperature below 60°.
10. A method according to claim 1 , further comprising an evaporating step after treating said surface with said ethylene oxide but before treating said surface with said hydrogen peroxide.
11. A method according to claim 1 , wherein the consumable in molecular biology is packaged beforehand in a partially nonwoven pack.
12. A method according to claim 1 , wherein vacuum is applied before treating said surface with said ethylene oxide or before treating said surface with said hydrogen peroxide.
13. A method according to claim 1 , wherein the residual ethylene oxide is eliminated after treating said surface with said ethylene oxide but before treating said surface with said hydrogen peroxide.
14. A method according to claim 1 , wherein the residual hydrogen peroxide is eliminated from the surface of the consumable after treating said surface with said hydrogen peroxide.
15. A consumable treated according to the method of claim 1 , wherein it is free of amplifiable residual nucleic acids.
16. A consumable according to claim 15 , wherein said consumable comprises a filter or a membrane, which filter or membrane is of polypropylene, PES or PVDF.