Method and apparatus for determining a focal position of an imaging device adapted to image a biologic sample
A method and apparatus for focusing a device for imaging a biologic sample is provided. A method aspect includes the steps of: disposing lenslets within a biologic sample, which lenslets have a height and a refractive index, which refractive index is different from that of the sample, wherein one or both of the imaging device and the sample are relatively locatable so a focal position of the imaging device can be moved along the height of the lenslets; imaging a portion of the sample including lenslets using transmittance at one or more wavelengths; determining an average light transmittance intensity of the sample at the wavelengths; determining an average light transmittance intensity of a region of each lenslet at the wavelengths; and determining the focal position of the imaging device using the average light transmittance intensity of the sample and the average light transmittance intensity of the region of the lenslets.
1. An analysis chamber for quiescently holding a biologic sample, which sample has a refractive index, comprising:
a first panel;
a second panel, at least one of which panels is transparent; and
a plurality of lenslets disposed and positionally fixed within the chamber between the first panel and the second panel, which lenslets have a height and a refractive index, which refractive index is different from that of the sample;
wherein each of the plurality of lenslets has a characteristic light transmittance pattern, wherein the characteristic light transmittance pattern is the same for each of the plurality of lenslets, wherein the characteristic light transmittance pattern is a function of the refractive index of the lenslets, and wherein the characteristic light transmittance patterns are repeatable and consistent amongst the plurality of lenslets; and
wherein the lenslets are all substantially the same shape.
2. The analysis chamber of claim 1 , wherein the lenslets are randomly positioned within the chamber.
3. The analysis chamber of claim 1 , wherein the lenslets are spherical.