Provided are bioactive compounds and metabolites derived from Chromobacterium species culture responsible for controlling pests, compositions containing these compounds, methods for obtaining these compounds and methods of using these compounds and compositions for controlling pests.
1. An isolated compound having the structure:
and which has insecticidal activity.
2. The isolated compound of claim 1 , wherein the isolated compound is isolated from a Chromobacterium species.
3. The isolated compound of claim 2 , wherein the Chromobacterium species is a Chromobacterium subtsugae.
4. The isolated compound of claim 3 , wherein the Chromobacterium subtsugae is Chromobacterium subtsugae sp. nov. (NRRL B-30655).
5. A composition comprising the isolated compound of claim 1 .
6. The composition of claim 5 , further comprising at least one of a carrier, diluent, surfactant, or adjuvant.
7. The composition of claim 5 , wherein the isolated compound is isolated from a Chromobacterium species.
8. The composition of claim 7 , wherein the Chromobacterium species is a Chromobacterium subtsugae.
9. The composition of claim 8 , wherein the Chromobacterium subtsugae is Chromobacterium subtsugae sp. nov. (NRRL B-30655).
10. A method for obtaining a compound from a Chromobacterium species comprising:
(a) culturing a strain of the Chromobacterium species in a whole cell broth under conditions sufficient to produce said compound; and
(b) isolating said compound produced in (a) from said whole cell broth, wherein the compound has a structure:
11. The method of claim 10 , wherein the Chromobacterium species is a Chromobacterium subtsugae.
12. The method of claim 11 , wherein the Chromobacterium subtsugae is Chromobacterium subtsugae sp. nov. (NRRL B-30655).
13. A method for modulating insect infestation in a plant comprising applying to the plant and/or seeds thereof and/or substrate used for growing said plant an amount of an isolated compound having the structure
and which has insecticidal activity, effective to modulate said insect infestation.
14. The method of claim 13 , wherein the isolated compound is isolated from a Chromobacterium species.
15. The method of claim 14 , wherein the Chromobacterium species is a Chromobacterium subtsugae.
16. The method of claim 15 , wherein the Chromobacterium subtsugae is Chromobacterium subtsugae sp. nov. (NRRL B-30655).
17. The method of claim 13 , wherein the isolated compound is applied to the substrate.
18. The method of claim 17 , wherein the substrate is soil.
19. The method of claim 13 , wherein the isolated compound is applied to the plant.
20. The method of claim 13 , wherein the isolated compound is applied to the seeds.